English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/174369
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Expression analysis of lung miRNAs responding to ovine VM virus infection by RNA-seq

AuthorsBilbao-Arribas, Martin; Abendaño, Naiara; Varela-Martínez, Endika; Reina, Ramsés ; Andrés, Damián F. de ; Jugo, Begoña M.
Host-virus interaction
Differential expression
Issue Date18-Jan-2019
PublisherBioMed Central
CitationBMC Genomics 20(1): 62 (2019)
Abstract[Background] MicroRNAs (miRNAs) are short endogenous, single-stranded, noncoding small RNA molecules of approximately 22 nucleotides in length. They regulate gene expression posttranscriptionally by silencing mRNA expression, thus orchestrating many physiological processes. The Small Ruminant Lentiviruses (SRLV) group includes the Visna Maedi Virus (VMV) and Caprine Arthritis Encephalitis (CAEV) viruses, which cause a disease in sheep and goats characterized by pneumonia, mastitis, arthritis and encephalitis. Their main target cells are from the monocyte/macrophage lineage. To date, there are no studies on the role of miRNAs in this viral disease.
[Results] Using RNA-seq technology and bioinformatics analysis, the expression levels of miRNAs during different clinical stages of infection were studied. A total of 212 miRNAs were identified, of which 46 were conserved sequences in other species but found for the first time in sheep, and 12 were completely novel. Differential expression analysis comparing the uninfected and seropositive groups showed changes in several miRNAs; however, no significant differences were detected between seropositive asymptomatic and diseased sheep. The robust increase in the expression level of oar-miR-21 is consistent with its increased expression in other viral diseases. Furthermore, the target prediction of the dysregulated miRNAs revealed that they control genes involved in proliferation-related signalling pathways, such as the PI3K-Akt, AMPK and ErbB pathways.
[Conclusions] To the best of our knowledge, this is the first study reporting miRNA profiling in sheep in response to SRLV infection. The known functions of oar-miR-21 as a regulator of inflammation and proliferation appear to be a possible cause of the lesions caused in the sheep’s lungs. This miRNA could be an indicator for the severity of the lung lesions, or a putative target for therapeutic intervention.
Publisher version (URL)https://doi.org/10.1186/s12864-018-5416-0
Appears in Collections:(IDAB) Artículos
Files in This Item:
File Description SizeFormat 
12864_2018_Article_5416.pdf2,03 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.