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Title

Acute stress regulates vasotocinergic and isotocinergic system in the gilthead sea bream (Sparus aurata)

AuthorsSkrzynska, Arleta K.; Maiorano, E.; Bastaroli, M.; Martínez-Rodríguez, Gonzalo CSIC ORCID ; Martos-Sitcha, Juan Antonio CSIC ORCID; Mancera, Juan Miguel
Issue DateSep-2015
Citation10º Congreso de la Asociación Ibérica de Endocrinología Comparada (2015)
AbstractThe hypothalamus-pituitary-interrenal axis is involved in stress response. Although corticotrophin releasing hormone (CRH) is considered the main hormone that activates this axis, other hormones as CRH binding protein (CRH-BP), arginine vasotocin (AVT) and isotocin (IT) are considered as important players in stress regulation. This study assessed, using the gilthead sea bream (Sparus aurata) as a biological model, hypothalamic mRNA expression changes of different endocrine factors (CRH, CRH-BP), as well as different neuropeptides (AVT e IT) and their receptors (AVTR V1-type, AVTR V2-type, and ITR) after an acute stress situation. Specimens were exposed to air for 3 min by lifting the wire- net cage out of the tank and put back in their respective tanks after that, being sampled in a time course response (15 min, 30 min, 1, 2, 4 and 8 hours post-stress). At hypothalamic level, acute stress affects mRNA expression of all precursors (pro-VT and pro-IT) and receptors (AVTRs and ITR), as well as hormonal factors (CRH and CRH-BP), by increasing and modulating their mRNA expression levels from the first 15 min post-emersion. Furthermore, our data provide evidence on existence of different variation pattern in the mRNA expression of both AVT receptors suggesting that each of them can be involved in different phases of stress response to cope with this adverse situation. Thus, the AVT V1-type receptor can be responsible for primary endocrine control while the AVTR V2-type can be involved in the regulation of several metabolic pathways to support energy demand enhancement required during stress processes. Our results demonstrate the activation of both endocrine pathways in the regulation of hypothalamic-pituitary-interrenal axis of S. aurata, which is stated, at least, through changes in mRNA expression levels of these genes.
DescriptionTrabajo presentado en el 10º Congreso de la Asociación Ibérica de Endocrinología Comparada, celebrado en Castellón del 23 al 25 de septiembre de 2015.
URIhttp://hdl.handle.net/10261/174248
Appears in Collections:(ICMAN) Comunicaciones congresos

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