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Evaluation of five serologic assays for bovine tuberculosis surveillance in domestic free-range pigs from southern Spain

AuthorsCardoso-Toset, Fernando; Luque, Inmaculada; Carrasco, Librado; Jurado-Martos, Francisco; Risalde, María Ángeles; Venteo, Ángel; Infantes-Lorenzo, José Antonio; Bezos, Javier; Rueda, Paloma; Tapia, Istar; Gortázar, Christian ; Domínguez, Lucas; Domínguez, Mercedes; Gomez-Laguna, Jaime
KeywordsTuberculosis-like lesions
Serologic assays
Issue Date2017
CitationPreventive Veterinary Medicine 137A: 101-104 (2017)
AbstractIn countries where bovine tuberculosis (bTB) is still prevalent the contact among different animal species in extensive systems contributes to the circulation of Mycobacterium bovis (M. bovis) and other members of the Mycobacterium tuberculosis complex (MTC). Thus, free-range pigs can develop subclinical infections and may contribute to disease spread to bovine and wildlife. Serodiagnosis has been proposed as a screening tool for detecting infected pig herds; however, the value of this method to obtain an accurate diagnosis in this species is still not clear. In this study, sensitivity (Se) and specificity (Sp) estimates of four ELISAs and a lateral flow immunochromatographic antibody assay based on different M. bovis antigens, including MPB70 and MPB83 proteins, were evaluated in naturally infected domestic free-range pigs. For this purpose, submandibular lymph nodes and blood samples from 217 pigs from both TB-infected and historically negative farms were sampled at slaughterhouse and analysed by gross examination, histopathology, bacteriological culture and qPCR. Se and Sp estimates of the 5 evaluated assays ranged from 66.1% to 78% (CI from 52.6 to 87.7%) and from 98.9% to 100% (CI from 93.8 to 100%), respectively. Results of our study suggest that all the evaluated assays could be used as a first screening tool to conduct bTB surveillance in domestic pigs at population level; however, animals from seropositive herds should later be surveyed by other methods in order to reduce false negative results.
Identifiersdoi: 10.1016/j.prevetmed.2016.12.016
issn: 0167-5877
e-issn: 1873-1716
Appears in Collections:(IREC) Artículos
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