Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/174200
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Título: | Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria |
Autor: | Sabater, Carlos CSIC ORCID ; Fara, Agustina; Palacios, Jorge; Corzo, Nieves CSIC ORCID ; Requena, Teresa CSIC ORCID ; Montilla, Antonia CSIC ORCID ; Zárate, Gabriela | Palabras clave: | Prebiotics Transgalactosylation Oligosaccharides β-galactosidase Propionibacteria |
Fecha de publicación: | 2019 | Editor: | Elsevier | Citación: | Food Microbiology 77: 93-105 (2019) | Resumen: | The potential of probiotic bacteria to produce prebiotic oligosaccharides by transgalactosylation has been minimally studied. In this work, we screened the β-galactosidase (β-gal) activity of dairy propionibacteria (PAB) isolated from Argentinean foods to select strains for the synthesis of oligosaccharides from lactose (GOS) and lactulose (OsLu). PAB, when grown in a medium with lactose as a carbon source, were disrupted, and the cell-free extracts were assayed for β-gal activity. Nine strains grew on lactose and showed β-gal activities from 0.27 to 2.60 U mL. Propionibacterium acidipropionici LET 120, the strain with the highest activity, was able to synthesize, using 30% lactose and lactulose at pH 6.5 and 45 °C, 26.8% of LET 120-GOS and 26.1% of LET 120-OsLu after 24 h. When they were tested as carbon sources for growth, P. acidipropionici LET 120 attained higher biomasses, μ and β-gal activities at the expense of Aspergillus oryzae-OsLu, Vivinal-GOS and lactulose compared to lactose or glucose. In addition, LET 120-GOS and LET 120-OsLu synthesized by PAB were prebiotic for some probiotic strains. For the first time, our results show the production of GOS and OsLu by dairy PAB, and these results encourage further studies on the optimization of the synthesis and structure characterization of the obtained oligosaccharides. | Versión del editor: | https://doi.org/10.1016/j.fm.2018.08.014 | URI: | http://hdl.handle.net/10261/174200 | DOI: | 10.1016/j.fm.2018.08.014 | Identificadores: | e-issn: 1095-9998 issn: 0740-0020 |
Aparece en las colecciones: | (CIAL) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
Sabater, 2019; Synthesis of prebiotic galactooligosaccharides from lactose and lactulose by dairy propionibacteria.pdf | 1,14 MB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
PubMed Central
Citations
3
checked on 08-abr-2024
SCOPUSTM
Citations
20
checked on 15-abr-2024
WEB OF SCIENCETM
Citations
16
checked on 29-feb-2024
Page view(s)
334
checked on 19-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
Artículos relacionados:
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.