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Title

In vitro and in situ techniques for estimating digestibility

AuthorsLópez, Secundino
KeywordsRumination
Digestion
Metabolism
Ruminants
Issue Date2005
PublisherCABI Publishing
CitationJ. Dijkstra, J.M. Forbes & J. France (eds.), Quantitative Aspects of Ruminant Digestion and Metabolism, 2nd edition pp. 87-121 (2005) CAB International, Wallingford, UK.
AbstractNew feeding systems need to be founded on the mechanisms that govern the response of animals to nutrients, dealing with quantitative aspects of the digestion and metabolism in the ruminant animal. Digestibility and rumen degradability have been recognized as the main sources of variation of the energy and protein value of feeds, respectively. For the quantitative description of digestive and metabolic processes, appropriate biological data are required and can be obtained using in vivo, in situ and in vitro methods. Information obtained in vivo is the most reliable and should be the reference to evaluate other methods, because it represents the actual animal response to a dietary treatment. However, in vivo digestion trials are expensive, laborious, time-consuming and not readily applicable to large numbers of feeds or when only small quantities of each feedstuff are available. In vivo results are restricted to the experimental conditions under which measurements are carried out, such as level of feeding and associative affects between feeds (Kitessa et al., 1999). In vivo techniques to determine rumen degradability or intestinal digestibility require animals surgically modified, and measurements of digesta flows and of microbial and endogenous contributions of nutrients may be needed, resulting in digestibility and degradability estimates subject to large variability and additional errors associated with use of digesta flow rate markers, microbial markers, and inherent animal variation. This variation demands use of sufficient experimental replication to obtain reliable results. Therefore, these trials cannot be considered routine in most laboratories...
Description36 páginas.--This chapter appeared in: Quantitative Aspects of Ruminant Digestion and Metabolism (4): 87-121 (2005)
Publisher version (URL)https://www.cabi.org/bookshop/book/9780851998145
http://dx.doi.org/10.1079/9780851998145.0049
URIhttp://hdl.handle.net/10261/174117
DOI10.1079/9780851998145.0049
ISBN0-85199-814-3
Appears in Collections:(IGM) Libros y partes de libros
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