English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/174024
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Oral administration of heat-inactivated Mycobacterium bovis reduces the response of farmed red deer to avian and bovine tuberculin

AutorLópez, Vladimir; González-Barrio, David ; Lima-Barbero, José Francisco; Ortiz, José-Antonio; Domínguez, Lucas; Juste, Ramón A.; Garrido, Joseba M.; Sevilla, Iker A.; Alberdi, Pilar ; Fuente, José de la ; Gortázar, Christian
Palabras claveRuminants
Complement component 3
Animal tuberculosis
Serum antibodies
Intradermal tuberculin test
Fecha de publicación2016
EditorElsevier
CitaciónVeterinary Immunology and Immunopathology 172: 21-25 (2016)
ResumenOrally delivered mycobacterial antigens may not sensitize the immunized animals causing a positive tuberculin skin test response. As the first step to address this critical issue, we characterized the response of farmed red deer (Cervus elaphus) to orally delivered heat-inactivated Mycobacterium bovis. Thirty-two adult red deer hinds from a farm known to be free of tuberculosis (TB) were randomly assigned to two different treatment groups, immunized (n = 24) and control (n = 8). Immunized hinds were dosed orally with 2 ml of PBS containing 6 × 10 heat-inactivated M. bovis. The mean skin test response of immunized deer to both avian purified protein derivative (aPPD) and bovine PPD (bPPD) was consistently lower in immunized than in control hinds. One year after immunization, immunized hinds had a significant reduction in the skin test response to aPPD and in the ELISA antibody levels against both aPPD and bPPD (24-36% reduction; P < 0.05). By contrast, no significant change was observed in the skin test response to phytohaemagglutinin, or in the ELISA antibody levels against the M. bovis specific antigen MPB70. The mRNA levels for C3, IFN-γ and IL-1β and serum protein levels for IFN-γ and IL-1β did not vary between immunized and control deer. However, serum C3 protein levels were significantly higher (P = 0.001) in immunized than in control deer six months after immunization. These results confirm that oral heat-inactivated M. bovis does not sensitize farmed red deer and therefore does not cause false-positive responses in the tuberculin skin test. The absence of sensitization in orally immunized deer opens the possibility of testing the vaccine in deer and possibly other ruminants without the risk of causing false-positive reactions in TB-tests. This study also provided the first evidence that orally-delivered inactivated mycobacterial antigens elicit some kind of immune response in a ruminant.
URIhttp://hdl.handle.net/10261/174024
Identificadoresdoi: 10.1016/j.vetimm.2016.03.003
e-issn: 1873-2534
issn: 0165-2427
Aparece en las colecciones: (IREC) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
accesoRestringido.pdf15,38 kBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.