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dc.contributor.authorGasol, Josep M.-
dc.contributor.authorZweifel, Ulla Li-
dc.contributor.authorPeters, Francesc-
dc.contributor.authorFuhrman, Jed A.-
dc.contributor.authorHagström, Åke-
dc.date.accessioned2019-01-03T13:02:41Z-
dc.date.available2019-01-03T13:02:41Z-
dc.date.issued1999-10-
dc.identifierissn: 0099-2240-
dc.identifiere-issn: 1098-5336-
dc.identifierdoi: 10.1128/AEM.65.10.4475-4483.1999-
dc.identifier.citationApplied and Environmental Microbiology 65(10): 4475-4483 (1999)-
dc.identifier.urihttp://hdl.handle.net/10261/173740-
dc.description9 pages, 6 figures, 4 tables-
dc.description.abstractTotal bacterial abundances estimated with different epifluorescence microscopy methods (4',6-diamidino-2-phenylindole [DAPI], SYBR Green, and Live/Dead) and with flow cytometry (Syto13) showed good correspondence throughout two microcosm experiments with coastal Mediterranean water. In the Syto13- stained samples we could differentiate bacteria with apparent high DNA (HDNA) content and bacteria with apparent low DNA (LDNA) content. HDNA bacteria, 'live' bacteria (determined as such with the Molecular Probes Live/Dead BatLight bacterial viability kit), and nucleoid-containing bacteria (NuCC) comprised similar fractions of the total bacterial community. Similarly, LDNA bacteria and 'dead' bacteria (determined with the kit) comprised a similar fraction of the total bacterial community in one of the experiments. The rates of change of each type of bacteria during the microcosm experiments were also positively correlated between methods. In various experiments where predator pressure on bacteria had been reduced, we detected growth of the HDNA bacteria without concomitant growth of the LDNA bacteria, such that the percentage contribution of HDNA bacteria to total bacterial numbers (%HDNA) increased. This indicates that the HDNA bacteria are the dynamic members of the bacterial assemblage. Given how quickly and easily the numbers of HDNA and LDNA bacteria can be obtained, and given the similarity to the numbers of 'live' cells and NuCC, the %HDNA is suggested as a reference value for the percentage of actively growing bacteria in marine planktonic environments-
dc.description.sponsorshipThis work was supported by two EU grants, MAS3-CT95-0016 (MEDEA) and MAR95-1901-C03-03 (MIDAS)-
dc.publisherAmerican Society for Microbiology-
dc.rightsclosedAccess-
dc.titleSignificance of Size and Nucleic Acid Content Heterogeneity as Measured by Flow Cytometry in Natural Planktonic Bacteria-
dc.typeartículo-
dc.identifier.doi10.1128/AEM.65.10.4475-4483.1999-
dc.relation.publisherversionhttps://doi.org/10.1128/AEM.65.10.4475-4483.1999-
dc.date.updated2019-01-03T13:02:41Z-
dc.description.versionPeer Reviewed-
dc.language.rfc3066eng-
dc.contributor.funderEuropean Commission-
dc.relation.csic-
dc.identifier.funderhttp://dx.doi.org/10.13039/501100000780es_ES
dc.type.coarhttp://purl.org/coar/resource_type/c_6501es_ES
item.openairetypeartículo-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextNo Fulltext-
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