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Title

Bacterial FtsZ protein forms phase-separated condensates with its nucleoid-associated inhibitor SlmA

AuthorsMonterroso, Begoña CSIC ORCID ; Zorrilla, Silvia CSIC ORCID ; Sobrinos-Sanguino, Marta CSIC ; Robles-Ramos, Miguel A. CSIC ; López-Álvarez, Marina; Margolin, William; Keating, Christine D.; Rivas, Germán CSIC ORCID
KeywordsBacterial division
Biomolecular condensation
Droplet microfluidics
Macromolecular crowding
Phase separation
Issue Date6-Dec-2018
PublisherEuropean Molecular Biology Organization
CitationEMBO Reports e45946 (2018)
AbstractMacromolecular condensation resulting from biologically regulated liquid-liquid phase separation is emerging as a mechanism to organize intracellular space in eukaryotes, with broad implications for cell physiology and pathology. Despite their small size, bacterial cells are also organized by proteins such as FtsZ, a tubulin homolog that assembles into a ring structure precisely at the cell midpoint and is required for cytokinesis. Here, we demonstrate that FtsZ can form crowding-induced condensates, reminiscent of those observed for eukaryotic proteins. Formation of these FtsZ-rich droplets occurs when FtsZ is bound to SlmA, a spatial regulator of FtsZ that antagonizes polymerization, while also binding to specific sites on chromosomal DNA. The resulting condensates are dynamic, allowing FtsZ to undergo GTP-driven assembly to form protein fibers. They are sensitive to compartmentalization and to the presence of a membrane boundary in cell mimetic systems. This is a novel example of a bacterial nucleoprotein complex exhibiting condensation into liquid droplets, suggesting that phase separation may also play a functional role in the spatiotemporal organization of essential bacterial processes.
Description13 p.-6 fig.
Publisher version (URL)https://doi.org/10.15252/embr.201845946
URIhttp://hdl.handle.net/10261/173643
DOI10.15252/embr.201845946
ISSN1469-221X
E-ISSN1469-3178
Appears in Collections:(CIB) Artículos

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