English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/173353
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Avenues to characterize the interactions of extended N-Glycans with proteins by NMR spectroscopy: The influenza hemagglutinin case

AuthorsFernández de Toro, Beatriz; Peng, Wenjie; Thompson, Andrew J.; Domínguez, Gemma; Cañada, F. Javier ; Pérez-Castells, Javier; Paulson, James C.; Jiménez-Barbero, Jesús ; Canales, Ángeles
KeywordsN-glycan
Hemagglutinin
Paramagnetic NMR
Pseudo contact shifts
Sialic acid
Issue Date12-Nov-2018
PublisherWiley-VCH
CitationAngew Chem Int Ed 57:15051 –15055 (2018)
AbstractLong-chain multiantenna N-glycans are extremely complex molecules. Their inherent flexibility and the presence of repetitions of monosaccharide units in similar chemical environments hamper their full characterization by X-ray diffraction or standard NMR methods. Herein, the successful conformational and interaction analysis of a sialylated tetradecasaccharide N-glycan presenting two LacNAc repetitions at each arm is presented. This glycan has been identified as the receptor of the hemagglutinin protein of pathogenic influenza viruses. To accomplish this study, a N-glycan conjugated with a lanthanide binding tag has been synthesized, enabling analysis of the system by paramagnetic NMR. Under paramagnetic conditions, the NMR signals of each sugar unit in the glycan have been determined. Furthermore, a detailed binding epitope of the tetradecasaccharide N-glycan in the presence of HK/68 hemagglutinin is described. © 2018 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
Description5 p.-2 fig.1 sch. Dedicated to Prof. Manuel Martín-Lomas on the occasion of his 77th birthday
Publisher version (URL)https://doi.org/10.1002/anie.201807162
https://doi.org/10.1002/ange.201807162
URIhttp://hdl.handle.net/10261/173353
DOI10.1002/anie.201807162
10.1002/ange.201807162
ISSN1433-7851
E-ISSN1521-3773
Appears in Collections:(CIB) Artículos
Files in This Item:
File Description SizeFormat 
Toro_et_al-2018-Angewandte_Chemie_International_Edition.pdfArtículo principal2,34 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.