English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/173163
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Título

Key Role of the Local Hydrophobicity in the East Patch of Plastocyanins on Their Thermal Stability and Redox Properties

AutorOlloqui-Sariego, José Luis; Marquez, I.; Frutos-Beltrán, Estrella ; Díaz-Moreno, Irene ; Rosa, Miguel A. de la ; Calvente, J.J.; Andreu, Rafael; Díaz-Quintana, Antonio
Fecha de publicación2018
EditorAmerican Chemical Society
CitaciónACS Omega 3: 11447- 11454 (2018)
ResumenUnderstanding the molecular basis of the thermal stability and functionality of redox proteins has important practical applications. Here, we show a distinct thermal dependence of the spectroscopic and electrochemical properties of two plastocyanins from the thermophilic cyanobacterium Phormidium laminosum and their mesophilic counterpart from Synechocystis sp. PCC 6803, despite the similarity of their molecular structures. To explore the origin of these differences, we have mimicked the local hydrophobicity in the east patch of the thermophilic protein by replacing a valine of the mesophilic plastocyanin by isoleucine. Interestingly, the resulting mutant approaches the thermal stability, redox thermodynamics, and dynamic coupling of the flexible site motions of the thermophilic protein, indicating the existence of a close connection between the hydrophobic packing of the east patch region of plastocyanin and the functional control and stability of the oxidized and reduced forms of the protein.
URIhttp://hdl.handle.net/10261/173163
Identificadoresdoi: 10.1021/acsomega.8b01612
issn: 2470-1343
Aparece en las colecciones: (IIQ) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
acsomega.8b01612.pdf4,33 MBAdobe PDFVista previa
Visualizar/Abrir
Mostrar el registro completo
 


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.