English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/173158
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Age-associated hydroxymethylation in human bone-marrow mesenchymal stem cells

AuthorsToraño, Estela G.; Bayón, Gustavo F.; Real, Álvaro del; Sierra, Marta I.; García, María G.; Carella, Antonella; Belmonte, Thalia; Urdinguio, Rocío G.; Cubillo, Isabel; García-Castro, Javier; Delgado-Calle, Jesús; Pérez-Campo, Flor M.; Riancho, José A.; Fraga, Mario F.; Fernández, Agustín F.
Issue Date2016
PublisherBioMed Central
CitationJournal of Translational Medicine 14: 207 (2016)
Abstract[Background]: Age-associated changes in genomic DNA methylation have been primarily attributed to 5-methylcytosine (5mC). However, the recent discovery of 5-hydroxymethylcytosine (5hmC) suggests that this epigenetic mark might also play a role in the process. [Methods]: Here, we analyzed the genome-wide profile of 5hmc in mesenchymal stem cells (MSCs) obtained from bone-marrow donors, aged 2-89 years. [Results]: We identified 10,685 frequently hydroxymethylated CpG sites in MSCs that were, as in other cell types, significantly associated with low density CpG regions, introns, the histone posttranslational modification H3k4me1 and enhancers. Study of the age-associated changes to 5hmC identified 785 hyper- and 846 hypo-hydroxymethylated CpG sites in the MSCs obtained from older individuals. [Conclusions]: DNA hyper-hydroxymethylation in the advanced-age group was associated with loss of 5mC, which suggests that, at specific CpG sites, this epigenetic modification might play a role in DNA methylation changes during lifetime. Since bone-marrow MSCs have many clinical applications, and the fact that the epigenomic alterations in this cell type associated with aging identified in this study could have associated functional effects, the age of donors should be taken into account in clinical settings.
Publisher version (URL)https://doi.org/10.1186/s12967-016-0966-x
Identifiersdoi: 10.1186/s12967-016-0966-x
e-issn: 1479-5876
Appears in Collections:(CINN) Artículos
Files in This Item:
File Description SizeFormat 
age-cell.pdf3,19 MBAdobe PDFThumbnail
Show full item record
Review this work

Related articles:

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.