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dc.contributor.authorQuirós del Bosque, Ana-
dc.contributor.authorRamos González, María Mercedes-
dc.contributor.authorMuguerza, B.-
dc.contributor.authorDelgado, M. A.-
dc.contributor.authorMartín-Álvarez, Pedro J.-
dc.contributor.authorAleixandre, Amaya-
dc.contributor.authorRecio, Isidra-
dc.date.accessioned2009-09-29T12:24:40Z-
dc.date.available2009-09-29T12:24:40Z-
dc.date.issued2006-12-
dc.identifier.citationJournal of Dairy Science 89(12): 4527-4535 (2006)en_US
dc.identifier.issn0022-0302-
dc.identifier.urihttp://hdl.handle.net/10261/17287-
dc.description9 páginas, 4 figuras, 3 tablas.-- PMID: 17106083 [PubMed].en_US
dc.descriptionEl artículo se puede acceder gratuitamente a través de la web del editor-
dc.description.abstractAmong different lactic acid bacteria isolated from raw milk, 4 Enterococcus faecalis strains have stood out as producers of fermented milk with potent antihyperten-sive activity. The peptide ß-casein f(133–138), LHLPLP, was identified as one of the major peptides responsible for the activity of these fermented milk products. A simple method was developed to quantify this peptide in fermented milk using high-performance liquid chromatography coupled in line with mass spectrometry. This procedure does not require any previous sample fractionation or extraction, and direct analysis of the water-soluble extract obtained from the fermented milk can be performed. Validation studies showed sufficient speci-ficity, reproducibility, linearity, and recovery, demonstrating that this method can be used for the routine quantification of LHLPLP during the production of fermented milk products. The developed method was readily applied to quantify the peptide LHLPLP under different fermentation conditions and with different aromatized products.en_US
dc.description.sponsorshipThis work has received financial support from the projects AGL2004-06903-C02-01 from the Ministerio de Educación y Ciencia, and CM-S0505-AGR-0153 financed by Comunidad de Madrid. A. Quirós was the recipient of a fellowship from the I3P Programme from the Consejo Superior de Investigaciones Científicas.en_US
dc.format.extent918459 bytes-
dc.format.mimetypeapplication/pdf-
dc.language.isoengen_US
dc.publisherAmerican Dairy Science Associationen_US
dc.rightsclosedAccessen_US
dc.subjectAntihypertensive peptidesen_US
dc.subjectEnterococcus faecalisen_US
dc.subjectQuantificationen_US
dc.subjectMass spectrometryen_US
dc.titleDetermination of the antihypertensive peptide LHLPLP in fermented milk by high-performance liquid chromatography-mass spectrometryen_US
dc.typeartículoen_US
dc.description.peerreviewedPeer revieweden_US
dc.relation.publisherversionhttp://www.journalofdairyscience.org/article/S0022-0302(06)72501-2/fulltexten_US
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