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Título

Determination of the antihypertensive peptide LHLPLP in fermented milk by high-performance liquid chromatography-mass spectrometry

AutorQuirós del Bosque, Ana; Ramos González, María Mercedes; Muguerza, B.; Delgado, M. A.; Martín-Álvarez, Pedro J. ; Aleixandre, Amaya; Recio, Isidra
Palabras claveAntihypertensive peptide
Enterococcus faecalis
Quantification
Mass spectrometry
Fecha de publicacióndic-2006
EditorAmerican Dairy Science Association
CitaciónJournal of Dairy Science 89(12): 4527-4535 (2006)
ResumenAmong different lactic acid bacteria isolated from raw milk, 4 Enterococcus faecalis strains have stood out as producers of fermented milk with potent antihyperten-sive activity. The peptide ß-casein f(133–138), LHLPLP, was identified as one of the major peptides responsible for the activity of these fermented milk products. A simple method was developed to quantify this peptide in fermented milk using high-performance liquid chromatography coupled in line with mass spectrometry. This procedure does not require any previous sample fractionation or extraction, and direct analysis of the water-soluble extract obtained from the fermented milk can be performed. Validation studies showed sufficient speci-ficity, reproducibility, linearity, and recovery, demonstrating that this method can be used for the routine quantification of LHLPLP during the production of fermented milk products. The developed method was readily applied to quantify the peptide LHLPLP under different fermentation conditions and with different aromatized products.
Descripción9 páginas, 4 figuras, 3 tablas.-- PMID: 17106083 [PubMed].
El artículo se puede acceder gratuitamente a través de la web del editor
Versión del editorhttp://www.journalofdairyscience.org/article/S0022-0302(06)72501-2/fulltext
URIhttp://hdl.handle.net/10261/17287
ISSN0022-0302
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