English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/172565
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:


Reverse Genetic Approaches for the Generation of Recombinant Zika Virus

AuthorsÁvila-Pérez, Ginés; Nogales, Aitor ; Martín, Verónica; Almazán, Fernando ; Martínez-Sobrido, Luis
Zika virus (ZIKV)
Reverse genetics
Infectious clone
Full-length molecular clone
Bacterial artificial chromosome
Infectious RNA
Issue Date31-Oct-2018
PublisherMultidisciplinary Digital Publishing Institute
CitationViruses 10(11): 597 (2018)
AbstractZika virus (ZIKV) is an emergent mosquito-borne member of the Flaviviridae family that was responsible for a recent epidemic in the Americas. ZIKV has been associated with severe clinical complications, including neurological disorder such as Guillain-Barré syndrome in adults and severe fetal abnormalities and microcephaly in newborn infants. Given the significance of these clinical manifestations, the development of tools and reagents to study the pathogenesis of ZIKV and to develop new therapeutic options are urgently needed. In this respect, the implementation of reverse genetic techniques has allowed the direct manipulation of the viral genome to generate recombinant (r)ZIKVs, which have provided investigators with powerful systems to answer important questions about the biology of ZIKV, including virus-host interactions, the mechanism of transmission and pathogenesis or the function of viral proteins. In this review, we will summarize the different reverse genetic strategies that have been implemented, to date, for the generation of rZIKVs and the applications of these platforms for the development of replicon systems or reporter-expressing viruses.
Publisher version (URL)https://doi.org/10.3390/v10110597
Appears in Collections:(CNB) Artículos
Files in This Item:
File Description SizeFormat 
viruses-10-00597.pdf1,61 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.