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Title

Reverse Genetic Approaches for the Generation of Recombinant Zika Virus

AuthorsÁvila-Pérez, Ginés; Nogales, Aitor ; Martín, Verónica; Almazán, Fernando ; Martínez-Sobrido, Luis
KeywordsFlavivirus
Zika virus (ZIKV)
Reverse genetics
Infectious clone
Full-length molecular clone
Bacterial artificial chromosome
Replicon
Infectious RNA
Issue Date31-Oct-2018
PublisherMultidisciplinary Digital Publishing Institute
CitationViruses 10(11): 597 (2018)
AbstractZika virus (ZIKV) is an emergent mosquito-borne member of the Flaviviridae family that was responsible for a recent epidemic in the Americas. ZIKV has been associated with severe clinical complications, including neurological disorder such as Guillain-Barré syndrome in adults and severe fetal abnormalities and microcephaly in newborn infants. Given the significance of these clinical manifestations, the development of tools and reagents to study the pathogenesis of ZIKV and to develop new therapeutic options are urgently needed. In this respect, the implementation of reverse genetic techniques has allowed the direct manipulation of the viral genome to generate recombinant (r)ZIKVs, which have provided investigators with powerful systems to answer important questions about the biology of ZIKV, including virus-host interactions, the mechanism of transmission and pathogenesis or the function of viral proteins. In this review, we will summarize the different reverse genetic strategies that have been implemented, to date, for the generation of rZIKVs and the applications of these platforms for the development of replicon systems or reporter-expressing viruses.
Publisher version (URL)https://doi.org/10.3390/v10110597
URIhttp://hdl.handle.net/10261/172565
DOI10.3390/v10110597
ISSN1999-4915
Appears in Collections:(CNB) Artículos
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