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Spectral, Photophysical, and Stability Properties of Isolated Photosystem II Reaction Center

AutorSeibert, Michael; Picorel Castaño, Rafael ; Rubin, Andrew B.; Connolly, John S.
Fecha de publicaciónjun-1988
EditorAmerican Society of Plant Biologists
CitaciónPlant Physiology 87:303-306 (1988)
ResumenPhotosystem II reaction center (RC) preparations isolated from spinach (Spinacea oleracea) by the Nanba-Satoh procedure (O Nanba, K Satoh 1987 Proc Natl Acad Sci USA 84: 109-112) are quite labile, even at 4°C in the dark. Simple spectroscopic criteria were developed to characterize the native state of the material. Degradation of the RC results in (a) blue-shifting of the red-most absorption maximum, (b) a shift of the 77 K fluorescence maximum from 682 nm to 670 nm, and (c) a shift of fluorescence lifetime components from 1.3-4 nanoseconds and >25 nanoseconds to 6-7 nanoseconds. Fluorescence properties at 77 K seem to be a more sensitive spectral indicator of the integrity of the material. The >25 nanosecond lifetime component is assigned to P680+ Pheophytin– recombination luminescence, which suggests a correlation between the observed spectral shifts and the photochemical competence of the preparation. Substitution of lauryl maltoside for Triton X-100 immediately after RC isolation stabilizes the RCs and suggests that Triton may be responsible for the instability.
Versión del editorhttp://www.plantphysiol.org/cgi/reprint/87/2/303
ISSN0032-0889 (Print)
1532-2548 (Online)
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