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Título

Stabilization of Isolated Photosystem II Reaction Center Complex in the Dark and in the Light Using Polyethylene Glycol and an Oxygen-Scrubbing System

AutorMcTavish, Hugh; Picorel Castaño, Rafael ; Seibert, Michael
Fecha de publicaciónfeb-1989
EditorAmerican Society of Plant Biologists
CitaciónPlant Physiology 89:452-456 (1989)
ResumenThe photosystem II reaction center as isolated (O Nanba, K Satoh [1987] Proc Natl Acad Sci USA 84: 109-112) is quite dilute and very unstable. Precipitating the complex with polyethylene glycol and resuspending it in buffer without detergent concentrates the reaction center and greatly improves its stability at 4°C in the dark as judged by light-induced electron transport activity. Furthermore, a procedure was developed to minimize photodestruction of polyethylene-glycol-concentrated material at room temperature in the light. The ability to stabilize the photosystem II reaction center should facilitate future photophysical, biochemical, and structural studies of the complex.
Versión del editorhttp://www.plantphysiol.org/cgi/reprint/89/2/452
URIhttp://hdl.handle.net/10261/17209
ISSN0032-0889 (Print)
1532-2548 (Online)
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