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Título: | Stabilization of Isolated Photosystem II Reaction Center Complex in the Dark and in the Light Using Polyethylene Glycol and an Oxygen-Scrubbing System |
Autor: | McTavish, Hugh; Picorel Castaño, Rafael CSIC ORCID ; Seibert, Michael | Fecha de publicación: | feb-1989 | Editor: | American Society of Plant Biologists | Citación: | Plant Physiology 89:452-456 (1989) | Resumen: | The photosystem II reaction center as isolated (O Nanba, K Satoh [1987] Proc Natl Acad Sci USA 84: 109-112) is quite dilute and very unstable. Precipitating the complex with polyethylene glycol and resuspending it in buffer without detergent concentrates the reaction center and greatly improves its stability at 4°C in the dark as judged by light-induced electron transport activity. Furthermore, a procedure was developed to minimize photodestruction of polyethylene-glycol-concentrated material at room temperature in the light. The ability to stabilize the photosystem II reaction center should facilitate future photophysical, biochemical, and structural studies of the complex. | Versión del editor: | https://doi.org/10.1104/pp.89.2.452 | URI: | http://hdl.handle.net/10261/17209 | ISSN: | 0032-0889 | E-ISSN: | 1532-2548 |
Aparece en las colecciones: | (EEAD) Artículos |
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