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Title

Genome-wide transcriptomic analysis of the Lactobacillus plantarum WCFS1 response to resveratrol

AuthorsReverón, Inés ; Franch, Mónica; Plaza-Vinuesa, Laura; Rivas, Blanca de las ; Muñoz, Rosario ; López de Felipe, Félix
KeywordsResveratrol
Transcriptomics
Issue Date2017
CitationBioMicroWorld 2017
AbstractResveratrol (RSV), a polyphenolic compound of plant origin, exerts many beneficial effects in humans and animal models but it displays low bioavailability in tissues involved in regulatory metabolism, such as visceral adipose tissues. However RSV accumulates in intestinal tissues suggesting that this polyphenol may exert its effects by modulating the gut microbiota. In spite RSV owns antimicrobial properties and has been reported to selectively increase Lactobacillus and Bifidobacterium populations in the gut, knowledge on the RSV tolerance mechanisms displayed by gut microorganisms is scarce. Hence, the objective of the present work was to study the mechanisms of adaptation of Lactobacillus plantarum WCFS1 to RSV. To this goal, we have investigated how RSV affects the expression profile of Lactobacillus plantarum WCFS1 at genome scale. Whole-transcriptome analysis was based on customized microarray profiles. Differentially expressed genes (fold-changes { 2 (p < 0.05)) were used to perform a functional analysis by using the DAVID bioinformatic tool. The transcriptomic datasets revealed the differential expression of a set of genes that highlight the genotoxicity of RSV to L. plantarum. The transcriptomic response also revealed differential expression of genes involved in the biosynthesis of cell wall components. Regarding the central metabolism, the expression profiles indicated tight control of ammonia levels and limitation of additional energy production. Among the RSV-responsive genes, several coding for transporters displayed marked variations in expression indicating that they might play significant roles for RSV tolerance in L. plantarum.
DescriptionPóster presentado a la VII International Conference on Environmental Industrial and Applied Microbiology, celebrada en Madrid (España) del 18 al 20 de octubre de 2017.
URIhttp://hdl.handle.net/10261/172022
Appears in Collections:(ICTAN) Comunicaciones congresos
(CNB) Comunicaciones congresos
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