English
español
Please use this identifier to cite or link to this item:
http://hdl.handle.net/10261/171927
Share/Impact:
Statistics |
![]() ![]() ![]() |
|
|
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |||
|
Title: | Solute carriers affect Anopheles stephensi survival and Plasmodium berghei infection in the salivary glands |
Authors: | Couto, Joana; Antunes, Sandra; Pinheiro-Silva, Renato; Rosário, V. do; Fuente, José de la ![]() |
Issue Date: | 2017 |
Publisher: | Nature Publishing Group |
Citation: | Scientific Reports 7(1): 6141 (2017) |
Abstract: | Malaria is caused by mosquito-borne Plasmodium spp. parasites that must infect and survive within mosquito salivary glands (SGs) prior to host transmission. Recent advances in transcriptomics and the complete genome sequencing of mosquito vectors have increased our knowledge of the SG genes and proteins involved in pathogen infection and transmission. Membrane solute carriers are key proteins involved in drug transport and are useful in the development of new interventions for transmission blocking. Herein, we applied transcriptomics analysis to compare SGs mRNA levels in Anopheles stephensi fed on non-infected and P. berghei-infected mice. The A. stephensi solute carriers prestinA and NDAE1 were up-regulated in response to infection. These molecules are predicted to interact with each other, and are reportedly involved in the maintenance of cell homeostasis. To further evaluate their functions in mosquito survival and parasite infection, these genes were knocked down by RNA interference. Knockdown of prestinA and NDAE1 resulted in reduction of the number of sporozoites in mosquito SGs. Moreover, NDAE1 knockdown strongly impacted mosquito survival, resulting in the death of half of the treated mosquitoes. Overall, our findings indicate the importance of prestinA and NDAE1 in interactions between mosquito SGs and Plasmodium, and suggest the need for further research. |
Publisher version (URL): | https://doi.org/10.1038/s41598-017-06317-6 |
URI: | http://hdl.handle.net/10261/171927 |
DOI: | 10.1038/s41598-017-06317-6 |
Identifiers: | doi: 10.1038/s41598-017-06317-6 e-issn: 2045-2322 |
Appears in Collections: | (IREC) Artículos |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
solutgland.pdf | 2,22 MB | Adobe PDF | ![]() View/Open |
Show full item record
Review this work
Review this work
Related articles:
WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.