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Delipidating mechanism of action in 3T3-L1 mature adipocytes treated with a combination of carob pod and wakame

AuthorsMiranda, J.; Rico, Daniel; Lasa, Arrate; Martínez Villaluenga, Cristina ; Martín Diana, Ana Belén; Arana, M.; Peñas, Elena
Issue Date2018
CitationXVII Congreso de la Sociedad Española de Nutrición (2018)
Abstract[Introduction]: Scientific research is constantly looking for new molecules that can be used as effective functional biomolecules in the fight against obesity and its co-morbidities. Our research teamhas previously reported the potential of Ceratonia siliqua L. (carob fruit) and Undaria pinnatifida (wakame seaweed) extracts in reducing triacylglycerol accumulation in mature adipocytes. [Objectives]: To analyze the effects of extracts from carob pod, wakame, and their combination on triacylglycerol (TG) accumulation in 3T3-L1 mature adipocytes, and to explore the mechanisms of action of this combination. [Methods]: Confluent 3T3-L1 pre-adipocytes were differentiated to mature by an induction media containing insulin, dexamethasone and 3-isobutyl-1-methylxanthine. After 10 days of differentiation, cells were treated with methanolic extracts of carob pod (50 mg/mL), wakame (1 mg/mL) and their combination. The maturation state for treatment was selected after performing a gene expression time course during the differentiation process. Cells were harvested 24 hours after the treatment and TG content was quantified. Total cell RNA was extracted with Trizol and ATGL (adipose triglyceride lipase), and HSL (hormone-sensitive lipase), ACC (acetyl-CoA carboxylase), FAS (fatty acid synthase), BCL-2 (B-cell lymphoma 2), BAX (Bcl-2-associated X protein), and gene expression was measured by real time polymerase chain reaction. [Results]: Wakame extract and its combination with carob pod decreased TG. Wakame reduced the HSL gene expression and increased BCL-2. In contrast, the combination diminished the FAS gene and raised BAX gene expression. No changes were observed in the remaining analyzed genes. [Conclusions]: The decrease in mature adipocyte triacylglycerol content induced by the combination of carob pod and wakame, but not by wakame alone, may be due, at least in part, to lipogenesis inhibition and apoptosis activation. Consequently, although further research is needed, this combination may be proposed as a potential fat-reduction inducer ingredient.
DescriptionResumen del póster presentado al XVII Congreso de la Sociedad Española de Nutrición [XVII Conference of the Spanish Nutrition Society (SEÑ) and the X Meeting of the Catalan Association of Food Science (ACCA)], celebrado en Barcelona (España) del 27 al 29 de junio de 2018.
Appears in Collections:(ICTAN) Comunicaciones congresos
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