English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/17155
Share/Impact:
Statistics
logo share SHARE   Add this article to your Mendeley library MendeleyBASE
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Title

Glutathione and Homoglutathione Synthesis in Legume Root Nodules

AuthorsMatamoros Galindo, Manuel Ángel ; Morán, José F. ; Iturbe-Ormaetxe, Iñaki ; Rubio Luna, María Carmen ; Becana Ausejo, Manuel
Issue DateNov-1999
PublisherAmerican Society of Plant Biologists
CitationPlant Physiol, November 1999, Vol. 121, pp. 879-888
AbstractHigh-performance liquid chromatography (HPLC) with fluorescence detection was used to study thiol metabolism in legume nodules. Glutathione (GSH) was the major non-protein thiol in all indeterminate nodules examined, as well as in the determinate nodules of cowpea (Vigna unguiculata), whereas homoglutathione (hGSH) predominated in soybean (Glycine max), bean (Phaseolus vulgaris), and mungbean (Vigna radiata) nodules. All nodules had greater thiol concentrations than the leaves and roots of the same plants because of active thiol synthesis in nodule tissue. The correlation between thiol tripeptides and the activities of glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) in the nodules of eight legumes, and the contrasting thiol contents and activities in alfalfa (Medicago sativa) leaves (98% hGSH, 100% hGSHS) and nodules (72% GSH, 80% GSHS) indicated that the distribution of GSH and hGSH is determined by specific synthetases. Thiol contents and synthesis decreased with both natural and induced nodule senescence, and were also reduced in the senescent zone of indeterminate nodules. Thiols and GSHS were especially abundant in the meristematic and infected zones of pea (Pisum sativum) nodules. Thiols and -glutamylcysteinyl synthetase were also more abundant in the infected zone of bean nodules, but hGSHS was predominant in the cortex. Isolation of full-length cDNA sequences coding for -glutamylcysteinyl synthetase from legume nodules revealed that they are highly homologous to those from other higher plants.
Publisher version (URL)http://www.plantphysiol.org/cgi/content/full/121/3/879
URIhttp://hdl.handle.net/10261/17155
ISSN0032-0889
E-ISSN1532-2548
Appears in Collections:(EEAD) Artículos
Files in This Item:
File Description SizeFormat 
MatamorosMA_PlantPhys_1999.pdf46,66 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.