English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/171463
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

The Role of CD38 on the Function of Regulatory B Cells in a Murine Model of Lupus

AuthorsBurlock, Brianna; Richardson, Gabrielle; García-Rodríguez, Sonia ; Guerrero, Salvador; Zubiaur, Mercedes; Sancho, Jaime
KeywordsBregs
CD38
CD1dhi
Lupus
IL-10
Autoimmunity
Inflammation
Issue Date25-Sep-2018
PublisherMultidisciplinary Digital Publishing Institute
CitationInternational Journal of Molecular Sciences 19(10): 2906 (2018)
AbstractPrevious work from our group has shown that Cd38−/− mice develop a milder pristane-induced lupus disease than WT or Art2−/− counterparts, demonstrating a new role for CD38 in promoting aberrant inflammation and lupus-like autoimmunity via a Transient Receptor Potential Melastatin 2 (TRPM2)-dependent apoptosis-driven mechanism. In this study we asked whether CD38 may play a role in the expression and function of regulatory B cells (IL-10-producing B cells or B10 cells). In pristane-treated mice the frequency of spleen CD19+CD1dhiCD5+ B cells, which are highly enriched in B10 cells, was significantly increased in Cd38−/− splenocytes compared to WT, while the frequency of peritoneal plasmacytoid dendritic cells (pDCs), which are major type I Interferon (IFN) producers, was greatly diminished. The low proportion of pDCs correlated with lower amounts of IFN-α in the peritoneal lavage fluids of the Cd38−/− mice than of WT and Art2−/− mice. Functional ex vivo assays showed increased frequencies of IL-10-producing B cells in Cd38−/− splenocytes than in WT upon stimulation with an agonist anti-CD40 mAb. Overall these results strongly suggest that Cd38−/− mice are better suited than WT mice to generate and expand regulatory B10 cells following the appropriate stimulation.
Publisher version (URL)https://doi.org/10.3390/ijms19102906
URIhttp://hdl.handle.net/10261/171463
DOI10.3390/ijms19102906
ISSN1661-6596
E-ISSN1422-0067
Appears in Collections:(IPBLN) Artículos
Files in This Item:
File Description SizeFormat 
ijms-19-02906.pdf2,3 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.