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Title

Beyond the outer limits of nature by directed evolution

AuthorsMolina-Espeja, Patricia CSIC ORCID ; Gómez-Fernández, Bernardo J.; Martín-Díaz, Javier ; García-Ruiz, Eva CSIC ORCID ; Alcalde Galeote, Miguel CSIC ORCID
KeywordsDirected evolution
Non-natural environments
Non-natural biocatalysis
Unnatural amino acids
Unnatural DNA
Gene therapy
Bioremediation
Biomedicine
Bionanomaterials
Issue DateOct-2016
PublisherElsevier
CitationBiotechnology Advances 34(5): 754-767 (2016)
AbstractFor more than thirty years, biotechnology has borne witness to the power of directed evolution in designing molecules of industrial relevance. While scientists all over the world discuss the future of molecular evolution, dozens of laboratory-designed products are being released with improved characteristics in terms of turnover rates, substrate scope, catalytic promiscuity or stability. In this review we aim to present the most recent advances in this fascinating research field that are allowing us to surpass the limits of nature and apply newly gained attributes to a range of applications, from gene therapy to novel green processes. The use of directed evolution in non-natural environments, the generation of catalytic promiscuity for non-natural reactions, the insertion of unnatural amino acids into proteins or the creation of unnatural DNA, is described comprehensively, together with the potential applications in bioremediation, biomedicine and in the generation of new bionanomaterials. These successful case studies show us that the limits of directed evolution will be defined by our own imagination, and in some cases, stretching beyond that.
Publisher version (URL)https://doi.org/10.1016/j.biotechadv.2016.03.008
URIhttp://hdl.handle.net/10261/171059
DOI10.1016/j.biotechadv.2016.03.008
ISSN0734-9750
Appears in Collections:(ICP) Artículos

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