English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/171010
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Title

Isolation of salt-tolerant mutants of Mesorhizobium ciceri strain Rch125 and identification of genes involved in salt sensitivity

AuthorsMoussaid, S.; Nogales, Joaquina; Muñoz, Socorro; Berraho, E.B.; Aurag, J.; Sanjuán, Juan
KeywordsChickpea
Mesorhizobium ciceri
Salt stress
Transposon mutagenesis
Issue Date2015
PublisherSpringer
CitationSymbiosis 67: 69- 77 (2015)
AbstractMesorhizobium ciceri Rch125 is a salt-sensitive strain isolated from root nodules of chickpea (Cicer arietinum L.). The aim of this work was to investigate the genes responsible for the sensitivity to salinity. Twelve Rch125 salt-tolerant mutants were isolated after random Tn5 mutagenesis and selected using a medium containing 300 mM NaCl, where growth of the wild-type is totally inhibited. In addition to this NaCl tolerance, the mutants also displayed higher tolerance to LiCl, CaCl and sucrose. Genes that were disrupted in the salt-tolerant mutants were in one of three functional categories: membrane transporters, outer membrane proteins, and genes of unknown function. Genetic complementation experiments demonstrated that the genes identified were involved in the salt sensitivity of the Rch125 strain. In most cases, disruption of the salt-sensitivity genes did not negatively affect the free-living or the symbiotic capabilities of Rch125 under non-saline conditions.
URIhttp://hdl.handle.net/10261/171010
Identifiersdoi: 10.1007/s13199-015-0357-8
issn: 1878-7665
Appears in Collections:(EEZ) Artículos
Files in This Item:
File Description SizeFormat 
accesoRestringido.pdf15,38 kBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.