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dc.contributor.authorSarasquete, Carmenes_ES
dc.contributor.authorÚbeda-Manzanaro, Maríaes_ES
dc.contributor.authorOrtiz-Delgado, Juan B.es_ES
dc.date.accessioned2018-10-11T11:24:44Z-
dc.date.available2018-10-11T11:24:44Z-
dc.date.issued2017-09-01-
dc.identifier.citationGeneral and Comparative Endocrinology 250: 136-151 (2017)-
dc.identifier.issn0016-6480-
dc.identifier.urihttp://hdl.handle.net/10261/170968-
dc.description.abstractThis study examines the effects induced by environmentally relevant concentrations of the isoflavone genistein (3 mg/L and 10 mg/L) during early life stages of the Senegalese sole. Throughout the hypothalamus-pituitary-thyroid (HPT) axis, several neurohormonal regulatory thyroid signalling patterns (thyroglobulin/Tg, thyroid peroxidase/TPO, transthyretin/TTR, thyroid receptors/TRβ, and iodothrynonine deiodinases, Dio2 and Dio3) were analysed. Furthermore, the expression patterns of estrogen receptor ERβ and haemoprotein Cyp1a were also evaluated. In the control larvae, progressive increases of constitutive hormonal signalling pathways have been evidenced from the pre-metamorphosis phase onwards, reaching the highest expression basal levels at the metamorphosis (Tg, TPO, Dio2) and/or during post-metamorphosis (TTR, TRβ, ERβ). When the early larvae were exposed to both genistein concentrations (3 mg/L and 10 mg/L), a statistically significant down-regulation of TPO, TTR and Tg mRNA levels was clearly detected at the metamorphic stages. In addition, the Dio2 and Dio3 transcript expression levels were also down and up-regulated when exposed to both genistein concentrations. In the larvae exposed to genistein, no statistically significant responses were recorded for the TRβ expression patterns. Nevertheless, the ERβ and Cyp1a transcript levels were up-regulated at the middle metamorphic stage (S2, at 16 dph) in the larvae exposed to high genistein concentrations and, only the ERβ was down-regulated (S1, at 12 dph) at the lower doses. Finally, all these pointed out imbalances were only temporarily disrupted by exposure to genistein, since most of the modulated transcriptional signals (i.e. up or down-regulation) were quickly restored to the baseline levels. Additionally, the control and genistein-exposed Senegalese sole specimens showed characteristic ontogenetic patterns and completely suitable for an optimal development, metamorphosis, and growth.es_ES
dc.description.sponsorshipThis study was financed by Spanish Project-MINECO-Feder (AGL2014-52906-R). Grant contract-CSIC (M. Úbeda-Manzanaro).es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.relationMINECO/ICTI2013-2016/AGL2014-52906-Res_ES
dc.rightsclosedAccesses_ES
dc.subjectCyp1aes_ES
dc.subjectEstrogenes_ES
dc.subjectGenisteines_ES
dc.subjectMetamorphosises_ES
dc.subjectSenegalese solees_ES
dc.subjectThyroides_ES
dc.titleEffects of the soya isoflavone genistein in early life stages of the Senegalese sole, Solea senegalensis: Thyroid, estrogenic and metabolic biomarkerses_ES
dc.typeartículoes_ES
dc.identifier.doi10.1016/j.ygcen.2017.06.008-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.ygcen.2017.06.008es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)es_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003339es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/501100003329es_ES
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