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Título

Blinking effect and the use of quantum dots in single molecule spectroscopy

AutorRombach-Riegraf, Verena; Oswald, Peter; Bienert, Roland; Petersen, Jan; Domingo, M.P.; Pardo, Julián; Gräber, P.; Gálvez Buerba, Eva Mª
Palabras claveSingle-molecule fluorescence
Quantum dot
Blinking
Cysteine
Protein
Fecha de publicación15-nov-2012
EditorElsevier
CitaciónBiochemical and Biophysical Research Communications
ResumenLuminescent semiconductor nanocrystals (quantum dots, QD) have unique photo-physical properties: high photostability, brightness and narrow size-tunable fluorescence spectra. Due to their unique properties, QD-based single molecule studies have become increasingly more popular during the last years. However QDs show a strong blinking effect (random and intermittent light emission), which may limit their use in single molecule fluorescence studies. QD blinking has been widely studied and some hypotheses have been done to explain this effect. Here we summarise what is known about the blinking effect in QDs, how this phenomenon may affect single molecule studies and, on the other hand, how the “on”/“off” states can be exploited in diverse experimental settings. In addition, we present results showing that site-directed binding of QD to cysteine residues of proteins reduces the blinking effect. This option opens a new possibility of using QDs to study protein–protein interactions and dynamics by single molecule fluorescence without modifying the chemical composition of the solution or the QD surface.
Versión del editorhttps://doi.org/10.1016/j.bbrc.2012.10.140
URIhttp://hdl.handle.net/10261/170486
DOI10.1016/j.bbrc.2012.10.140
ISSN0006-291X
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