Por favor, use este identificador para citar o enlazar a este item:
http://hdl.handle.net/10261/170364
COMPARTIR / EXPORTAR:
SHARE CORE BASE | |
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE | |
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.contributor.author | Viguera, Enrique | es_ES |
dc.contributor.author | Hernandez, Pablo | es_ES |
dc.contributor.author | Krimer, Dora B. | es_ES |
dc.contributor.author | Boistov, Alexander S. | es_ES |
dc.contributor.author | Lurz, Rudi | es_ES |
dc.contributor.author | Alonso, Juan Carlos | es_ES |
dc.contributor.author | Schvartzman, Jorge Bernardo | es_ES |
dc.date.accessioned | 2018-09-28T11:44:56Z | - |
dc.date.available | 2018-09-28T11:44:56Z | - |
dc.date.issued | 1996-09-13 | - |
dc.identifier.citation | J Biol Chem 271(37):22414-21 (1996) | es_ES |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.uri | http://hdl.handle.net/10261/170364 | - |
dc.description | 9 p.-8 fig. | es_ES |
dc.description.abstract | Co-orientation of replication origins is the most common organization found in nature for multimeric plasmids. Streptococcus pyogenes broad-host-range plasmid pSM19035 and Escherichia coli pPI21 are among the exceptions. pPI21, which is a derivative of pSM19035 and pBR322, has two long inverted repeats, each one containing a potentially active ColE1 unidirectional origin. Analysis of pPI21 replication intermediates (RIs) by two-dimensional agarose gel electrophoresis and electron microscopy revealed the accumulation of a specific RI containing a single internal bubble. The data obtained demonstrated that initiation of DNA replication occurred at a single origin in pPI21. Progression of the replicating fork initiated at either of the two potential origins was transiently stalled at the other inversely oriented silent ColE1 origin of the plasmid. The accumulated RIs, containing an internal bubble, occurred as a series of stereoisomers with different numbers of knots in their replicated portion. These observations provide one of the first functional explanations for the disadvantage of head-to-head plasmid multimers with respect to head-to-tail ones. | es_ES |
dc.description.sponsorship | This work was partially supported by Grants 93/0161 and 96/0470 from the Spanish Fondo de Investigación Sanitaria and Grant AE00118/94 from the Comunidad Autónoma de Madrid, Madrid, Spain. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Biochemistry and Molecular Biology | es_ES |
dc.relation.isversionof | Publisher's version | es_ES |
dc.rights | openAccess | es_ES |
dc.subject | Ribosomal-rna genes | es_ES |
dc.subject | SV40 DNA replication | es_ES |
dc.subject | Intertwined catenated dimers | es_ES |
dc.subject | Agarose-gel electrophoresis | es_ES |
dc.subject | Papillomavirus type-1 DNA | es_ES |
dc.subject | Escherichia-coli | es_ES |
dc.subject | Saccharomyces-cerevisiae | es_ES |
dc.subject | Plasmid replication | es_ES |
dc.subject | Potential origins | es_ES |
dc.subject | Bacillus-subtilis | es_ES |
dc.title | The ColE1 unidirectional origin acts as a polar replication fork pausing site | es_ES |
dc.type | artículo | es_ES |
dc.identifier.doi | 10.1074/jbc.271.37.22414 | - |
dc.description.peerreviewed | Peer reviewed | es_ES |
dc.relation.publisherversion | http://dx.doi.org/10.1074/jbc.271.37.22414 | es_ES |
dc.identifier.e-issn | 1083-351X | - |
dc.contributor.funder | Instituto de Salud Carlos III | es_ES |
dc.contributor.funder | Comunidad de Madrid | es_ES |
dc.relation.csic | Sí | es_ES |
oprm.item.hasRevision | no ko 0 false | * |
dc.identifier.funder | http://dx.doi.org/10.13039/501100004587 | es_ES |
dc.identifier.funder | http://dx.doi.org/10.13039/100012818 | es_ES |
dc.contributor.orcid | Viguera, Enrique [0000-0001-5475-3807] | es_ES |
dc.contributor.orcid | Hernandez, Pablo [0000-0002-8113-1277] | es_ES |
dc.contributor.orcid | Krimer, Dora B. [0000-0001-9972-3994] | es_ES |
dc.contributor.orcid | Alonso, Juan Carlos [0000-0002-5178-7179] | es_ES |
dc.type.coar | http://purl.org/coar/resource_type/c_6501 | es_ES |
item.openairetype | artículo | - |
item.grantfulltext | open | - |
item.cerifentitytype | Publications | - |
item.openairecristype | http://purl.org/coar/resource_type/c_18cf | - |
item.fulltext | With Fulltext | - |
item.languageiso639-1 | en | - |
Aparece en las colecciones: | (CIB) Artículos (CNB) Artículos |
Ficheros en este ítem:
Fichero | Descripción | Tamaño | Formato | |
---|---|---|---|---|
J. Biol. Chem.-1996-Viguera-22414-21.pdf | Artículo principal | 571,57 kB | Adobe PDF | Visualizar/Abrir |
CORE Recommender
SCOPUSTM
Citations
44
checked on 17-abr-2024
WEB OF SCIENCETM
Citations
45
checked on 26-feb-2024
Page view(s)
270
checked on 22-abr-2024
Download(s)
185
checked on 22-abr-2024
Google ScholarTM
Check
Altmetric
Altmetric
NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.