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Design of oligonucleotide-capped mesoporous silica nanoparticles for the detection of miRNA-145 by duplex and triplex formation

AutorRibes, Àngela; Santiago-Felipe, Sara; Aviñó, Anna; Candela-Noguera, Vicente; Eritja Casadellà, Ramón; Sancenón, Félix; Martínez-Mañez, Ramón; Aznar, Elena
Palabras claveMesoporous silica materials
Molecular gates
miRNA-145
Biosensor
Triplex
Fecha de publicacióndic-2018
EditorElsevier
CitaciónSensors and Actuators - B - Chemical Biochemical Sensors 277: 598-603 (2018)
ResumenThe development of new strategies to detect microRNAs (miRNAS) has become an important challenge in the biomedical field. We report herein the use of oligonucleotide-gated silica nanoparticles for the detection of miRNA-145. In the proposed design, mesoporous silica nanoparticles (MSNs) are loaded with a fluorescent reporter (rhodamine B) and pores are blocked by specific DNA oligonucleotides. The opening of the gated system and dye delivery is selectively controlled by DNA-miRNA recognition. Moreover, the use of DNA capture probes able to form duplex or triplex structures between target miRNA and the complementary oligonucleotides has been studied. By this simple procedure a limit of detection as low as 0.25 pM was found for miRNA. The method was successfully applied to detect miRNA-145 in serum samples, which demonstrates the high potential of these capped materials to detect miRNA for diagnostic purposes.
Versión del editorhttps://doi.org/10.1016/j.snb.2018.09.026
URIhttp://hdl.handle.net/10261/170312
DOI10.1016/j.snb.2018.09.026
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