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Biosensing at disk microelectrode arrays. Inter-electrode functionalization allows formatting into miniaturised sensing platforms of enhanced sensitivity.

AuthorsBaldrich, Eva; Campo García, Francisco Javier del ; Muñoz Pascual, Francisco Javier
microelectrode arrays
horse radish peroxidase
Issue Date16-Sep-2009
AbstractBiosensor performance depends on the effective functionalisation of a transducer with suitable biorecognition elements. During functionalisation, surface blocking steps are normally carried out to avoid later binding of undesirable molecules and thus guarantee biosensor specificity. However, these blocking steps may be deleterious in electrochemical systems where transduction ultimately relies on electron transfer between the electrode and a redox species in solution. This work presents a novel approach to develop improved amperometric biosensing platforms using microfabricated disk microelectrode arrays, based on the functionalisation of the inert surface surrounding the active microdisks. These devices more than doubled assay sensitivity compared to conventional biosensors produced using the same arrays. This approach benefits from three advantages: the functionalisation of a broader surface, the possibility to activate the microelectrodes immediately before detection, and access to enhanced rates of mass transport to microelectrodes that improve device sensitivity. To demonstrate this, we first studied the electrochemical behaviour of tetramethylbenzidine (TMB) at gold disk microelectrode arrays, and then used TMB as the redox mediator for the amperometric biosensing of HRP/H2O2. Down to 0.54 pM H2O2 or as little as 25 pM HRP were detected within 5 seconds of enzyme activity in just 10 μl of enzyme substrate solution. We postulate that microelectrode arrays may be used to develop novel electrochemical biosensing platforms that are faster and more sensitive than conventional biosensors.
DescriptionEn este trabajo demostramos el uso de redes de microelectrodos como base para el desarrollo de biosensores. La principal ventaja está en que las biomoléculas empleadas para reconocer los analitos se inmovilizan en la parte inerte de la red. Es decir, en el espacio entre microelectrodos. De este modo los electrodos pueden mantenerse activos y conservar su alta sensibilidad.
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