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Title

Characterizing the Transcriptional Effects of Endolysin Treatment on Established Biofilms of Staphylococcus aureus

AuthorsFernández, Lucía; González, Silvia; Gutiérrez, Diana ; Campelo, Ana B. ; Martínez Fernández, Beatriz ; Rodríguez González, Ana ; García Suárez, María Pilar
KeywordsBiofilms
Endolysins
Staphylococcus aureus
RNA-seq
Responses to antimicrobials
Issue Date20-Jun-2018
PublisherBio-protocol
CitationBio-Protocol 8(12): e2891 (2018)
AbstractBiofilms are the most common lifestyle of bacteria in both natural and human environments. The organized structure of these multicellular communities generally protects bacterial cells from external challenges, thereby enhancing their ability to survive treatment with antibiotics or disinfectants. For this reason, the search for new antibiofilm strategies is an active field of study. In this context, bacteriophages (viruses that infect bacteria) and their derived proteins have been proposed as promising alternatives for eliminating biofilms. For instance, endolysins can degrade peptidoglycan and, ultimately, lyse the target bacterial cells. However, it is important to characterize the responses of bacterial cells exposed to these compounds in order to improve the design of phage-based antimicrobial strategies.
This protocol was developed to examine the transcriptional responses of Staphylococcus aureus biofilm cells exposed to endolysin treatment, as previously described in Fernández et al. (2017). However, it may be subsequently adapted to analyze the response of other microorganisms to different antimicrobials.
Publisher version (URL)https://doi.org/10.21769/BioProtoc.2891
URIhttp://hdl.handle.net/10261/170038
DOI10.21769/BioProtoc.2891
E-ISSN2331-8325
Appears in Collections:(IPLA) Artículos
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