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dc.contributor.authorCeballos-Laita, Lauraes_ES
dc.contributor.authorGutiérrez-Carbonell, Elaínes_ES
dc.contributor.authorImai, Hiroyukies_ES
dc.contributor.authorAbadía Bayona, Anunciaciónes_ES
dc.contributor.authorUemura, Matsuoes_ES
dc.contributor.authorAbadía Bayona, Javieres_ES
dc.contributor.authorLópez-Millán, Ana Flores_ES
dc.identifier.citationCeballos-Laita L, Gutiérrez-Carbonell E, Imai H, Abadía A, Uemura M, Abadía J, López-Millán AF. Effects of manganese toxicity on the protein profile of tomato (Solanum lycopersicum) roots as revealed by two complementary proteomic approaches, two-dimensional electrophoresis and shotgun analysis. Journal of Proteomics 185: 51-63 (2018)es_ES
dc.description13 Pags.- 3 Tabls.- 4 Figs.es_ES
dc.description.abstractThe aim of this work was to assess the effects of manganese (Mn) toxicity on the proteome of tomato roots using two proteomic approaches, shotgun and two-dimensional electrophoresis. The shotgun approach yielded 367 reliable proteins, whereas the 2-DE approach detected 340 consistent spots. The 2-DE method found 54 proteins changing in relative abundance in the excess Mn treatment, whereas the shotgun detected changes in 118 proteins. Only 7% of the differential proteins were found by both methods, illustrating their complementary nature. Metabolic pathways most affected were protein metabolism, oxido-reductases and signaling. Results support that Mn toxicity alters the protein turnover and impairs energy production in roots, leading to changes in glycolysis, pyruvate metabolism, TCA and oxidative phosphorylation. Excess Mn also induced changes in peroxidases and hydrolases participating in cell wall lignification and suberization and activated plant defense mechanisms, with changes occurring via pathogenesis-related proteins as well as peroxidases. Finally, Mn toxicity elicited regulatory mechanisms and affected the abundance of root nutrient reservoir proteins. The overall analysis of the differential root proteome upon Mn toxicity suggests a general slowdown of metabolic activities, especially energy production, cell wall integrity and protein turnover, which occurs in parallel with increases in stress related proteins.es_ES
dc.description.sponsorshipSupported by the Spanish Ministry of Economy and Competitiveness (MINECO; project AGL2016-75226-R, co-financed with FEDER). Research conducted in Iwate University was in part supported by JSPS KAKENHI Grant Numbers JP24-7373, JP22120003, and JP24370018. E.G.-C. was supported by a JAE Pre-CSIC contract and L.C.-L was supported by a FPI-MINECO contract.es_ES
dc.subjectMn toxicityes_ES
dc.titleEffects of manganese toxicity on the protein profile of tomato (Solanum lycopersicum) roots as revealed by two complementary proteomic approaches, two-dimensional electrophoresis and shotgun analysises_ES
dc.description.peerreviewedPeer reviewedes_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderEuropean Commissiones_ES
dc.contributor.funderIwate Universityes_ES
dc.contributor.funderConsejo Superior de Investigaciones Científicas (España)es_ES
oprm.item.hasRevisionno ko 0 false*
dc.contributor.orcidCeballos-Laita, Laura [0000-0002-7223-1719]es_ES
dc.contributor.orcidAbadía Bayona, Anunciación [0000-0003-3609-7070]es_ES
dc.contributor.orcidUemura, Matsuo [0000-0003-0436-2976]es_ES
dc.contributor.orcidAbadía Bayona, Javier [0000-0001-5470-5901]es_ES
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