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Evaluating tolerance to NaCl in Vitis vinifera ssp. sylvestris by in vitro culture of buds

AutorGuerra Jiménez, Patricia
Palabras claveWild grapevine
Salt tolerance
In vitro culture
Ramsey rootstock
Fecha de publicación12-jul-2016
EditorCSIC - Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS)
Universidad de Sevilla
CitaciónEvaluating tolerance to NaCl in Vitis vinifera ssp. sylvestris by in vitro culture of buds. 1-20 (2016)
ResumenThe cultivation of the vine is subjected to significant genetic erosion driven by the introduction of foreign vine varieties, commercially more interesting. This decrease in biodiversity dangerously enhances the vulnerability of these vineyards situations, hitherto unknown and expected due to global change, such as pests, diseases or sensitivity to abiotic stresses, increasing saline soils. In vitro plant tissue culture allows to obtain a large number of healthy plants, both genetically and physiologically homogeneous. Moreover, being a closed system, plants can be selected according to their response to several factors including salt stress. In this work the in vitro bud culture is used to compare, according to their salt tolerance (NaCl), plants of two Andalusian wild grapevine populations, CO7 and J3, and the rootstock Ramsey considered very tolerant to salt. The results show a good response of explants from the three accessions to the micropropagation system used with a high survival percentage, always superior than 93%, in conditions without salt. On the other hand, the parameters analyzed along the study allow establishing the tolerance threshold at 2.5 gL-1 NaCl. CO7 shows higher carotenoids contents and lower MDA level than J3 and Ramsey. The results indicate that it is possible to classify the accessions according to salt tolerance as CO7>J3>Ramsey. And support the in vitro culture as a suitable method for early assessment of saline effects on plant material vine.
DescripciónDirectores del Proyecto: Cantos, Manuel ; García Fernández, José Luis-- Tutor: García González, Mercedes
URIhttp://hdl.handle.net/10261/169799
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