English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/169682
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Oxidative stress in retinal pigment epithelium cells increases exosome secretion and promotes angiogenesis in endothelial cells.

AutorAtienzar‐Aroca, Sandra; Flores‐Bellver, Miguel; Serrano‐Heras, Gemma; Martinez‐Gil, Natalia; Barcia, Jorge M.; Aparicio, Silvia ; Perez‐Cremades, Daniel; Garcia‐Verdugo, Jose M.; Diaz‐Llopis, Manuel; Romero, Francisco J.; Sancho‐Pelluz, Javier
Palabras claveVEGF receptors
Oxidative stress
Retinal pigment epithelium
Fecha de publicaciónago-2016
CitaciónJournal of Cellular and Molecular Medicine
ResumenThe retinal pigment epithelium (RPE), a monolayer located between the photoreceptors and the choroid, is constantly damaged by oxidative stress, particularly because of reactive oxygen species (ROS). As the RPE, because of its physiological functions, is essential for the survival of the retina, any sustained damage may consequently lead to loss of vision. Exosomes are small membranous vesicles released into the extracellular medium by numerous cell types, including RPE cells. Their cargo includes genetic material and proteins, making these vesicles essential for cell-to-cell communication. Exosomes may fuse with neighbouring cells influencing their fate. It has been observed that RPE cells release higher amounts of exosomes when they are under oxidative stress. Exosomes derived from cultured RPE cells were isolated by ultracentrifugation and quantified by flow cytometry. VEGF receptors (VEGFR) were analysed by both flow cytometry and Western blot. RT-PCR and qPCR were conducted to assess mRNA content of VEGFRs in exosomes. Neovascularization assays were performed after applying RPE exosomes into endothelial cell cultures. Our results showed that stressed RPE cells released a higher amount of exosomes than controls, with a higher expression of VEGFR in the membrane, and enclosed an extra cargo of VEGFR mRNA. Angiogenesis assays confirmed that endothelial cells increased their tube formation capacity when exposed to stressed RPE exosomes.
Descripción10 páginas, 5 figuras
Versión del editorhttp://dx.doi.org/10.1111/jcmm.12834
Aparece en las colecciones: (IBV) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
2016 J Cell Mol Med 20-1457.pdf820,48 kBAdobe PDFVista previa
Mostrar el registro completo

Artículos relacionados:

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.