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The activity of the ribosome synthesis pathway during proliferation. Causes and consequences of nucleolar stress

AutorDosil, Mercedes
Fecha de publicación2016
EditorSociedad Española de Bioquímica y Biología Molecular
CitaciónXXXIX Congreso de la SEBBM (2016)
ResumenRibosome synthesis takes place within the most prominent nuclear substructure, the nucleolus. It is a multistep process that entails pre-rRNA transcription, pre-rRNA processing, and assembly of ribosomal proteins imported from the cytoplasm. These activities require the participation of more than 200 trans-acting factors. The majority of studies on ribosome formation have been performed in yeast, and it has long been assumed that most features are similar in humans. However, although the basic aspects are conserved, there are many maturation events, and links to other processes, that are unique of higher eukaryotes. One such link is the nucleolar stress response, which is mediated by p53, and is triggered by alterations of ribosome synthesis. This response is receiving intense attention because it is the cause of various human diseases known as ribosomopathies. Despite this growing interest, progress in understanding ribosome synthesis in humans is greatly hampered by technical limitations. Powerful approaches used in yeast, such as genetic screens to identify trans-acting factors or purification of pre-ribosomes by tandem-affinity chromatography, are not possible in human cells. In our laboratory we have optimized a series of techniques to monitor the formation of different pre-ribosomal complexes in human cells. We are studying normally-growing cells and cells blocked at specific steps of ribosome maturation. Data will be shown on the defects in ribosome synthesis that occur in Diamond-Blackfan anemia and on the mechanisms underlying p53 activation in this ribosomopathy.
DescripciónResumen del póster presentado al XXXIX Congreso de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Salamanca del 5 al 8 de septiembre de 2016.
Aparece en las colecciones: (IBMCC) Comunicaciones congresos
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