English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/169473
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:

Identification and metagenetic characterisation of Listeria monocytogenes-harbouring communities present in food-related industrial environments

AutorRodríguez-López, P. ; Bernárdez Costas, Marta ; Rodríguez Herrera, Juan José ; Comesaña, Ángel Sebastián; López Cabo, Marta
Palabras claveBacterial communities
Food industry
Food safety
High throughput sequencing
Listeria monocytogenes
Fecha de publicación2019
CitaciónFood Control 95: 6-17 (2019)
ResumenThe main aim of this study was to localise, identify and characterise the Listeria monocytogenes-harbouring bacterial communities present in food related premises via 16S rRNA gene metagenetic analysis. With this scope, 319 environmental samples coming from a wide variety of surfaces of fish (n = 120), meat (n = 80) and dairy industries (n = 119), were firstly analysed following ISO 11290–1 and ISO 11290–2 norms. Direct L. monocytogenes quantification was possible in 9 samples (2.8%) with values between 2.00 and 3.97 log CFU/cm2. After enrichment, an overall L. monocytogenes incidence of 12.54% (n = 40) was obtained, being samples from meat industry the most contaminated. Molecular serotyping assays showed that most of the isolates belonged to 1/2b-3b-7 subgroup, followed by 1/2a-3a and 1/2c-3c. These results combined with AscI and ApaI PFGE macrorestriction patterns, yielded 7 different L. monocytogenes clusters. Nevertheless, no clear ecological relationships could be stablished. High amounts of L. monocytogenes-associated psychrotrophic microbiota were obtained in all cases with values above 9 log CFU/cm2 in some cases. Metagenetic analysis of one representative sample per each food industry type (fish, meat, dairy) demonstrated that Actinobacteria (53.16%) was mostly present in the meat sample whereas Proteobacteria was the most representative phylum in dairy (69.58%) and fish (97.11%) samples. Subsequent operational taxonomic units (OTUs) analysis, showed a wide variety of taxa associated with L. monocytogenes such as spoilage-associated genera (e.g. Psyschromonas or Shewanella), lactic acid bacteria genera (e.g. Lactococcus or Lactobacillus) or pathogenic species such as Yersinia enterocolitica. It was thus demonstrated, that L. monocytogenes is capable to both survive with different bacteria in different ecological niches, highlighting once more the need for proper surveillance schedules so as to guarantee the safety of the food products
Descripción12 pages, 6 figures, 3 tables
Versión del editorhttps://doi.org/10.1016/j.foodcont.2018.07.023
Aparece en las colecciones: (IIM) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Identification_metagenetic_2019.pdf Embargado hasta 1 de enero de 2020Article824,56 kBAdobe PDFVista previa
Visualizar/Abrir     Petición de una copia
Identification_metagenetic_Supp_Table1.xlsx Embargado hasta 1 de enero de 202036,84 kBMicrosoft Excel XMLVisualizar/Abrir     Petición de una copia
Identification_metagenetic_Supp_Table2_R2.xlsx Embargado hasta 1 de enero de 202022,36 kBMicrosoft Excel XMLVisualizar/Abrir     Petición de una copia
Identification_metagenetic_Supp_able3.docx Embargado hasta 1 de enero de 202013,06 kBMicrosoft Word XMLVisualizar/Abrir     Petición de una copia
Mostrar el registro completo

NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.