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Chromatin dynamics in response to DNA damage

AutorCampillo-Marcos, Ignacio ; Salzano, Marcella ; García González, Raúl; Lazo, Pedro A.
Fecha de publicación2016
EditorSociedad Española de Bioquímica y Biología Molecular
CitaciónXXXIX Congreso SEBBM (2016)
Resumen[Introduction]: Genome integrity is continuously challenged by exogenous and endogenous agents causing DNA damage, both in euchromatin and heterocromatin. The repair of these lesions involves changes in the histone organization, such as recruitment and/or incorporation of histone variants or covalent modifications of histones, in order to promote the formation of open, relaxed chromatin structues. Recently, a growing number of chromatin components, remodelers and modifications has been identificated as key players in DNA repair, emphasizing the complex role of chromatin in this process. On the other hand, defects in DNA repair pathways enable cancer cells to survive DNA damage. For this reason, the combination of DNA-damaging agents with specific inhibitors of these pathways could be used in cancer treatment. Currently, specific inhibitors against chromatin remodelers have been developed, but it is still unclear how they act in tumor cells. Our aim ls to detemine the effect of histone acetylation/deacetylation and methylation/demethylation inhibition on DNA repair foci. [Results and conclusion]: We use pharmacological inhibitors of HAIs (C646 and MGl49); HDACs (Entinostat and SAHA); KMTs (Chaetocin); and KDMs (JMJD2 inhibitor). Some of them are being employed in preclinical regulatory studies. In this analysis, we can observe that closed chromatin induced by HAIs inhibitors seems to affect the formation of yH2AX and 53BP1 foci, and the dynamics offoci formation corresponds with the increase of fluorescence level of H4 acetylation after inducing DNA damage. Based on these results, we conclude that chromatin relaxation is an essential early step in DNA repair, which can be blocked by specific inhibitors against HAIs, sensibilizing cells to treatments
DescripciónResumen del póster presentado al XXXIX Congreso de la Sociedad Española de Bioquímica y Biología Molecular, celebrado en Salamanca del 5 al 8 de septiembre de 2016.
Aparece en las colecciones: (IBMCC) Comunicaciones congresos
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