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Antimicrobial activity of binary combinations of natural and synthetic phenolic antioxidants against Enterococcus faecalis

AuthorsGutiérrez-Fernández, Javier; García-Armesto, M.R.; Álvarez-Alonso, R.; del Valle, P.; de Arriaga, D.; Rúa, J.
Issue Date2013
PublisherAmerican Dairy Science Association
CitationJournal of Dairy Science 96: 4912- 4920 (2013)
AbstractThis study investigated the antimicrobial activity of 3 natural (thymol, carvacrol, and gallic acid) and 2 synthetic [butylated hydroxyanisole (BHA) and octyl gallate] phenolic compounds, individually and in binary combinations, on 4 dairy isolates of Enterococcus faecalis with different virulence factors (β-hemolytic, gelatinase, or trypsin activities; acquired resistance to erythromycin or tetracycline; and natural resistance to gentamicin). A checkerboard technique and a microdilution standardized method were used. All compounds individually tested exhibited antimicrobial activity against E. faecalis, with minimal inhibitory concentrations (MIC) ranging from 30. μg/mL (octyl gallate) to 3,150. μg/mL (gallic acid), although no significant differences were detected among strains to each phenolic compound. Carvacrol in combination with thymol or gallic acid, and gallic acid combined with octyl gallate showed partial synergistic inhibition of all E. faecalis strains. The most effective combinations were thymol. +. carvacrol and gallic acid. +. octyl gallate, as the MIC for each of these compounds was reduced by 67 to 75% compared with their respective individual MIC. These results highlight the possibility of using combinations of these phenolic compounds to inhibit the growth of potential virulent or spoilage E. faecalis strains by reducing the total amount of additives used in dairy foods. © 2013 American Dairy Science Association.
Identifiersdoi: 10.3168/jds.2013-6643
issn: 0022-0302
Appears in Collections:(IQFR) Artículos
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