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Título

Introduction to the diagnosis and classification of monocytic-lineage leukemias by flow cytometry

AutorMatarraz, Sergio; Almeida, Julia ; Flores-Montero, Juan; Lécrevisse, Quentin; Guerri, Valentina; López, Antonio; Barrena, Susana; Velden, Vincent H. J. van der; Marvelde, Jeroen G. te; Dongen, J. J. M. van; Orfao, Alberto
Palabras claveAcute leukemia
Monocytes
Flow cytometry
Fecha de publicación2017
EditorJohn Wiley & Sons
CitaciónCytometry Part B: Clinical Cytometry 92(3): 218-227 (2017)
ResumenDespite diagnostic criteria are currently available for the distinct subtypes of monocytic-lineage neoplasias, a number of partially overlapping features still remain evident, which may hamper their differential diagnosis. An accurate identification and characterization of monocytic cells is of major relevance for the diagnosis and classification of these neoplasias. In this regard, as compared to other conventional techniques, flow cytometry has shown the highest sensitivity for detection of early monocytic commitment of (normal and neoplastic) bone marrow CD34 hematopoietic precursors as well as of monocytic aberrations and maturation blockades, which are frequently associated with clonal myeloid disorders. In the present paper we provide basic principles and criteria for multiparameter flow cytometry identification and characterization of bone marrow monocytic cells that contribute to an improved diagnostic and classification of monocytic lineage-associated acute leukemias in clinical settings, particularly when using the EuroFlow antibody panels.
URIhttp://hdl.handle.net/10261/169165
Identificadoresdoi: 10.1002/cyto.b.21219
e-issn: 1552-4957
issn: 1552-4949
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