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Studies towards hcTnI Immunodetection Using Electrochemical Approaches Based on Magnetic Microbeads

AuthorsHernández-Albors, Alejandro; Colom, Gloria; Salvador, Juan Pablo; Marco, María Pilar
KeywordsHuman cardiac troponin I
Magnetic beads
Cadmium quantum dots
Streptavidin-Horseradish Peroxidase
Issue Date29-Jul-2018
PublisherMultidisciplinary Digital Publishing Institute
CitationSensors 18(8): 2457 (2018)
AbstractDifferent electrochemical strategies based on the use of magnetic beads are described in this work for the detection of human cardiac troponin I (hcTnI). hcTnI is also known as the gold standard for acute myocardial infarction (AMI) diagnosis according to the different guidelines from the European Society of Cardiology (ESC) and the American College of Cardiology (ACC). Amperometric and voltamperometric sandwich magnetoimmunoassays were developed by biofunctionalization of paramagnetic beads with specific antibodies. These bioconjugates were combined with biotinylated antibodies as detection antibodies, with the aim of testing different electrochemical transduction principles. Streptavidin labeled with horseradish peroxidase was used for the amperometric magnetoimmunoassay, reaching a detectability of 0.005 ± 0.002 µg mL−1 in 30 min. Cadmium quantum dots-streptavidin bioconjugates were used in the case of the voltamperometric immunosensor reaching a detectability of 0.023 ± 0.014 µg mL−1
Publisher version (URL)https://doi.org/10.3390/s18082457
Appears in Collections:(IQAC) Artículos
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