English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/169011
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:


Seasonal and Sexual Differences in the Microbiota of the Hoopoe Uropygial Secretion

AuthorsRodríguez-Ruano, Sonia M.; Martín-Vivaldi, Manuel; Peralta-Sánchez, Juan M. ; García-Martín, Ana B. ; Martínez-García, Ángela; Soler, Juan José ; Valdivia, Eva; Martínez-Bueno, Manuel
Fluorescence in situ hybridization (FISH)
High-throughput sequencing
Quantitative polymerase chain reaction (qPCR)
Uropygial gland secretion
Issue Date11-Aug-2018
PublisherMultidisciplinary Digital Publishing Institute
CitationGenes 9(8): 407 (2018)
AbstractThe uropygial gland of hoopoe nestlings and nesting females hosts bacterial symbionts that cause changes in the characteristics of its secretion, including an increase of its antimicrobial activity. These changes occur only in nesting individuals during the breeding season, possibly associated with the high infection risk experienced during the stay in the hole-nests. However, the knowledge on hoopoes uropygial gland microbial community dynamics is quite limited and based so far on culture-dependent and molecular fingerprinting studies. In this work, we sampled wild and captive hoopoes of different sex, age, and reproductive status, and studied their microbiota using quantitative polymerase chain reaction (qPCR), fluorescence in situ hybridization (FISH) and pyrosequencing. Surprisingly, we found a complex bacterial community in all individuals (including non-nesting ones) during the breeding season. Nevertheless, dark secretions from nesting hoopoes harbored significantly higher bacterial density than white secretions from breeding males and both sexes in winter. We hypothesize that bacterial proliferation may be host-regulated in phases of high infection risk (i.e., nesting). We also highlight the importance of specific antimicrobial-producing bacteria present only in dark secretions that may be key in this defensive symbiosis. Finally, we discuss the possible role of environmental conditions in shaping the uropygial microbiota, based on differences found between wild and captive hoopoes.
Publisher version (URL)https://doi.org/10.3390/genes9080407
Appears in Collections:(EEZA) Artículos
Files in This Item:
File Description SizeFormat 
genes-09-00407-v2.pdf2,38 MBAdobe PDFThumbnail
Show full item record
Review this work

WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.