English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/16887
Compartir / Impacto:
Estadísticas
Add this article to your Mendeley library MendeleyBASE
Citado 15 veces en Web of Knowledge®  |  Pub MebCentral Ver citas en PubMed Central  |  Ver citas en Google académico
Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Título

Effect of triploidy on turbot haematology

AutorCal, Rosa; Vidal, S.; Camacho, T.; Piferrer, Francesc ; Guitián, F.J.
Palabras claveTurbot
Triploidy
Blood
Erythrocyte
Haematology
Haemoglobin
Oxygen transport
Fecha de publicaciónmay-2005
EditorElsevier
CitaciónComparative Biochemistry and Physiology Part A 141(1): 35-41 (2005)
ResumenThis study was carried out to compare key haematological features of diploid (2n) and triploid (3n) turbot as a first step towards the assessment of the ability of 3n turbot to withstand sub-optimal culture conditions. Morphometric indices of erythrocytes were determined on blood smears by light microscopy. Triploidy significantly (P < 0.001) increased all morphometric indices measured in the erythrocytes, including size, surface, and volume, except for the size of minor nuclear axis. The increase in cell size was larger for the major (31.0%) than for the minor (8.3%) axis, thus rendering erythrocytes of 3n turbot more ellipsoidal. The increase in erythrocyte volume (45.9%) was close to the theoretical expected 50% increase as a result of one extra chromosome set. Haematological indices were measured automatically by a haematological Coulter Counter. Triploid turbot had lower numbers of red blood cells (RBC: 1.84 cells pL−1 in 2n vs. 1.27 cells pL−1 in 3n; P < 0.001) but they were of a larger size (Mean corpuscular volume [MCV]: 145.51 fL in 2n vs. 181.78 fL in 3n; P < 0.001). However, the decrease in RBC was not compensated by the increase in MCV, and thus, triploidy decreased the haematocrit (Hct: 26.80% in 2n vs. 23.11% in 3n; P < 0.001) and total blood haemoglobin concentration (Hb: 73.74 g l−1 in 2n vs. 67.54 g l−1 in 3n; P < 0.05). In contrast, mean corpuscular hemoglobin (MCH: 40.27 pg in 2n vs. 53.28 pg in 3n; P < 0.001) was higher for 3n turbot as a result of their larger erythrocytes although MCH concentration (MCHC: 0.28 pg fL−1 in 2n vs. 0.29 pg fL−1 in 3n did not significantly differ. RBC, Hct and MCV were also determined manually using light microscopy. In general, discrepancies between the two methods were small (overall not, vert, similar7%) but the Coulter Counter tended to overestimate RBC and Hct (and thus to underestimate MCV). Nevertheless, relative differences between ploidies were very similar, thus verifying triploidy-associated changes in hematological features. These changes, as determined in the present study, are essential when evaluating the feasibility of triploid turbot for intensive aquaculture systems in which unfavorable situations may occur.
Descripción7 pages, 2 figures, 3 tables.-- PMID: 15914050 [PubMed].
Versión del editorhttp://dx.doi.org/10.1016/j.cbpb.2005.03.007
URI10261/16887
DOI10.1016/j.cbpb.2005.03.007
ISSN1095-6433
Aparece en las colecciones: (ICM) Artículos
Ficheros en este ítem:
No hay ficheros asociados a este ítem.
Mostrar el registro completo
 



NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.