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dc.contributor.authorPuig-Kröger, Amayaes_ES
dc.contributor.authorLópez-Rodríguez, Cristinaes_ES
dc.contributor.authorRelloso, Migueles_ES
dc.contributor.authorSánchez-Elsner, Tilmanes_ES
dc.contributor.authorNueda, Arsenioes_ES
dc.contributor.authorMuñoz, Eduardoes_ES
dc.contributor.authorBernabéu, Carmeloes_ES
dc.contributor.authorCorbí, Angel L.es_ES
dc.date.accessioned2018-08-17T09:16:51Z-
dc.date.available2018-08-17T09:16:51Z-
dc.date.issued2000-09-15-
dc.identifier.citationJ Biol Chem 275(37):28507-12 (2000)es_ES
dc.identifier.issn0021-9258-
dc.identifier.urihttp://hdl.handle.net/10261/168807-
dc.description7 p.-7 fig.1 tab.es_ES
dc.description.abstractThe CD11a/CD18 leukocyte integrin (LFA-1; also known as alphaL/beta2) mediates leukocyte transendothelial migration during immune and inflammatory responses and participates in lymphoma metastasis. CD11a/CD18 leukocyte-restricted expression is controlled by the CD11a gene promoter, which confers tissue-specific expression to reporter genes in vitro and in vivo. DNase I protection analysis of the CD11a proximal gene promoter revealed DNA-protein interactions centered at position -110 (CD11a-110). Disruption of CD11a-110 reduced CD11a promoter activity in a cell type-specific manner, as it reduced its activity by 70% in Jurkat lymphoid cells, whereas the effect was considerably lower in K562 and HepG2 cells. Electrophoretic mobility shift assays showed evidence of cell type-specific differences in CD11a-110 binding and indicated its specific recognition by members of the polyomavirus enhancer-binding protein 2/core binding factor (CBF)/acute myeloid leukemia (AML) family of transcription factors. AML1B/CBFbeta transactivated the CD11a promoter, with AML1B/CBFbeta-mediated transactivation being completely dependent on the integrity of the CD11a-110 element. Therefore, CBF/AML factors play a role in the cell type-restricted transcription of the CD11a integrin gene through recognition of CD11a-110. The involvement of CBF/AML factors in CD11a expression raises the possibility that CD11a/CD18 expression might be deregulated in acute myeloid and B-lineage acute lymphoblastic leukemias, thus contributing to their altered adhesion and metastatic potential.es_ES
dc.description.sponsorshipThis work was supported by Comision Interministerial de Cienca Y Tecnologia (CICYT) Grant SAF98/0068 and Comunidad Autónoma de Madrid Grant 08.2/0035.1/99 (to A. L. C.) and by CICYT Grant SAF97/0034 (to C. B.).es_ES
dc.language.isoenges_ES
dc.publisherAmerican Society for Biochemistry and Molecular Biologyes_ES
dc.relation.isversionofPublisher's versiones_ES
dc.rightsopenAccesses_ES
dc.subjectFunction-associated antigen-1es_ES
dc.subjectNuclear factor-ies_ES
dc.subjectTranscription factores_ES
dc.subjectMurine myeloperoxidasees_ES
dc.subjectAdhesion moleculeses_ES
dc.subjectExpressiones_ES
dc.subjectIdentificationes_ES
dc.subjectCloninges_ES
dc.subjectlfa-1es_ES
dc.subjectLeukemogenesises_ES
dc.titlePolyomavirus enhancer-binding protein 2/core binding factor/acute myeloid leukemia factors contribute to the cell type-specific activity of the CD11a integrin gene promoteres_ES
dc.typeartículoes_ES
dc.identifier.doihttp://dx.doi.org/10.1074/jbc.M004323200-
dc.description.peerreviewedPeer reviewedes_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1074/jbc.M004323200es_ES
dc.identifier.e-issn1083-351X-
dc.contributor.funderComisión Interministerial de Ciencia y Tecnología, CICYT (España)es_ES
dc.contributor.funderComunidad de Madrides_ES
dc.relation.csices_ES
oprm.item.hasRevisionno ko 0 false*
dc.identifier.funderhttp://dx.doi.org/10.13039/501100007273es_ES
dc.identifier.funderhttp://dx.doi.org/10.13039/100012818es_ES
dc.contributor.orcidPuig-Kröger, Amaya [0000-0003-2943-9757]es_ES
dc.contributor.orcidLópez-Rodríguez, Cristina [0000-0002-2311-2406]es_ES
dc.contributor.orcidRelloso, Miguel [0000-0002-9181-2244]es_ES
dc.contributor.orcidSánchez-Elsner, Tilman [0000-0003-1915-2410]es_ES
dc.contributor.orcidNueda, Arsenio [0000-0001-8373-500X]es_ES
dc.contributor.orcidBernabéu, Carmelo [0000-0002-1563-6162]es_ES
dc.contributor.orcidCorbí, Angel L.[0000-0003-1980-5733]es_ES
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