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A putative RNA binding protein from Plasmodium vivax apicoplast

AuthorsGarcía-Mauriño, Sofía M.; Díaz-Quintana, Antonio; Rivero-Rodríguez, Francisco; Cruz-Gallardo, Isabel CSIC ORCID; Grüttner, Christian; Hernández-Vellisca, Marian; Díaz-Moreno, Irene CSIC ORCID
KeywordsApicoplast RNA binding protein
Protein aggregation
Malarial Plasmodium parasite
Issue Date2018
PublisherFederation of European Biochemical Societies
CitationFEBS Open Bio 8: 177- 188 (2018)
AbstractMalaria is caused by Apicomplexa protozoans from the Plasmodium genus entering the bloodstream of humans and animals through the bite of the female mosquitoes. The annotation of the Plasmodium vivax genome revealed a putative RNA binding protein (apiRBP) that was predicted to be trafficked into the apicoplast, a plastid organelle unique to Apicomplexa protozoans. Although a 3D structural model of the apiRBP corresponds to a noncanonical RNA recognition motif with an additional C-terminal ¿-helix (¿3), preliminary protein production trials were nevertheless unsuccessful. Theoretical solvation analysis of the apiRBP model highlighted an exposed hydrophobic region clustering ¿3. Hence, we used a C-terminal GFP-fused chimera to stabilize the highly insoluble apiRBP and determined its ability to bind U-rich stretches of RNA. The affinity of apiRBP toward such RNAs is highly dependent on ionic strength, suggesting that the apiRBP-RNA complex is driven by electrostatic interactions. Altogether, apiRBP represents an attractive tool for apicoplast transcriptional studies and for antimalarial drug design.
Identifiersdoi: 10.1002/2211-5463.12351
issn: 2211-5463
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