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Purification and structural analysis of plectin and BPAG1e

AutorManso, José A. ; García-Rubio, Inés; Gómez-Hernández, María ; Ortega, Esther ; Buey, Ruben M.; Carballido Vázquez, Ana M. ; Carabias, Arturo ; Alonso-García, Noelia ; Pereda, José M. de
Palabras claveRecombinant expression
Spectrin repeat
Hybrid methods
DEER
SAXS
Crystallography
Protein structure
Plakin
Fecha de publicación2016
EditorElsevier
CitaciónMethods in Enzymology 569: 177-196 (2016)
ResumenPlectin and BPAG1e belong to the plakin family of high-molecular-weight proteins that interconnect the cytoskeletal systems and anchor them to junctional complexes. Plectin and BPAG1e are prototypical plakins with a similar tripartite modular structure. The N- and C-terminal regions are built of multiple discrete structural domains, while the central rod domain mediates dimerization by coiled-coil interactions. Owing to the mosaic organization of plakins, the structure of their constituent individual domains or small multi-domain segments can be analyzed isolated. Yet, understanding the integrated function of large regions, oligomers, and heterocomplexes of plakins is difficult due to the large and segmented structure. Here, we describe methods for the production of plectin and BPAG1e samples suitable for structural and biophysical analysis. In addition, we discuss the combination of hybrid methods that yield information at several resolution levels to study the complex, multi-domain, and flexible structure of plakins.
URIhttp://hdl.handle.net/10261/168689
ISBN978-0-12-803469-9
Identificadoresdoi: 10.1016/bs.mie.2015.05.002
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