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Insights into the human mesenchymal stromal/stem cell identity through integrative transcriptomic profiling

AutorRosón, Beatriz ; Sánchez-Guijo, Fermín M.; Cañizo, María Consuelo del; De Las Rivas, Javier
Palabras claveCD marker
Bone marrow
Stromal cells
Adipose tissue
Human gene expression
Bioinformatic meta-analysis
Placenta
Cytokines
Mesenchymal stem cells
Fecha de publicación2016
EditorBioMed Central
CitaciónBMC Genomics 17(1): 944 (2016)
Resumen[Background]: Mesenchymal Stromal/Stem Cells (MSCs), isolated under the criteria established by the ISCT, still have a poorly characterized phenotype that is difficult to distinguish from similar cell populations. Although the field of transcriptomics and functional genomics has quickly grown in the last decade, a deep comparative analysis of human MSCs expression profiles in a meaningful cellular context has not been yet performed. There is also a need to find a well-defined MSCs gene-signature because many recent biomedical studies show that key cellular interaction processes (i.e. inmuno-modulation, cellular cross-talk, cellular maintenance, differentiation, epithelial-mesenchymal transition) are dependent on the mesenchymal stem cells within the stromal niche. [Results]: In this work we define a core mesenchymal lineage signature of 489 genes based on a deep comparative analysis of multiple transcriptomic expression data series that comprise: (i) MSCs of different tissue origins; (ii) MSCs in different states of commitment; (iii) other related non-mesenchymal human cell types. The work integrates several public datasets, as well as de-novo produced microarray and RNA-Seq datasets. The results present tissue-specific signatures for adipose tissue, chorionic placenta, and bone marrow MSCs, as well as for dermal fibroblasts; providing a better definition of the relationship between fibroblasts and MSCs. Finally, novel CD marker patterns and cytokine-receptor profiles are unravelled, especially for BM-MSCs; with MCAM (CD146) revealed as a prevalent marker in this subtype of MSCs. [Conclusions]: The improved biomolecular characterization and the released genome-wide expression signatures of human MSCs provide a comprehensive new resource that can drive further functional studies and redesigned cell therapy applications.
Versión del editorhttps://doi.org/10.1186/s12864-016-3230-0
URIhttp://hdl.handle.net/10261/168604
Identificadoresdoi: 10.1186/s12864-016-3230-0
issn: 1471-2164
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