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Title

Genomic resources for a commercial flatfish, the Senegalese sole (Solea senegalensis): EST sequencing, oligo microarray design, and development of the Soleamold bioinformatic platform

AuthorsCerdà, Joan ; Mercadé, Jaume; Lozano, Juan José; Manchado, Manuel; Tingaud-Sequeira, Angèle; Astola, A. ; Infante, Carlos; Halm, Silke ; Viñas, Jordi ; Castellana, Bárbara; Asensio, Esther; Cañavate, J. Pedro; Martínez-Rodríguez, Gonzalo ; Piferrer, Francesc ; Planas, Josep V.; Prat, Francisco ; Yúfera, Manuel ; Durany, Olga; Subirada, Francesc; Rosell, Elisabet; Maes, Tamara
Issue Date30-Oct-2008
PublisherBioMed Central
CitationBMC Genomics 9: 508 (2008)
Abstract[Background] The Senegalese sole, Solea senegalensis, is a highly prized flatfish of growing commercial interest for aquaculture in Southern Europe. However, despite the industrial production of Senegalese sole being hampered primarily by lack of information on the physiological mechanisms involved in reproduction, growth and immunity, very limited genomic information is available on this species.
[Results] Sequencing of a S. senegalensis multi-tissue normalized cDNA library, from adult tissues (brain, stomach, intestine, liver, ovary, and testis), larval stages (pre-metamorphosis, metamorphosis), juvenile stages (post-metamorphosis, abnormal fish), and undifferentiated gonads, generated 10,185 expressed sequence tags (ESTs). Clones were sequenced from the 3'-end to identify isoform specific sequences. Assembly of the entire EST collection into contigs gave 5,208 unique sequences of which 1,769 (34%) had matches in GenBank, thus showing a low level of redundancy. The sequence of the 5,208 unigenes was used to design and validate an oligonucleotide microarray representing 5,087 unique Senegalese sole transcripts. Finally, a novel interactive bioinformatic platform, Soleamold, was developed for the Senegalese sole EST collection as well as microarray and ISH data.
[Conclusion] New genomic resources have been developed for S. senegalensis, an economically important fish in aquaculture, which include a collection of expressed genes, an oligonucleotide microarray, and a publicly available bioinformatic platform that can be used to study gene expression in this species. These resources will help elucidate transcriptional regulation in wild and captive Senegalese sole for optimization of its production under intensive culture conditions.
Description14 pages, 5 figures, 3 tables.-- PMID: 18973667 [PubMed].-- PMCID: PMC2612027.-- Supporting information available at: http://www.biomedcentral.com/1471-2164/9/508/additional/
Publisher version (URL)http://dx.doi.org/10.1186/1471-2164-9-508
URIhttp://hdl.handle.net/10261/16855
DOI10.1186/1471-2164-9-508
ISSN1471-2164
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