English   español  
Please use this identifier to cite or link to this item: http://hdl.handle.net/10261/168397
Share/Impact:
Statistics
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL | DATACITE
Exportar a otros formatos:

Title

Vitamin A Affects Flatfish Development in a Thyroid Hormone Signaling and Metamorphic Stage Dependent Manner

AuthorsFernández, Ignacio; Ortiz-Delgado, Juan B. CSIC ORCID ; Darias, M. J. CSIC ORCID; Hontoria, Francisco CSIC ORCID ; Andree, Karl B.; Manchado, Manuel; Sarasquete, Carmen CSIC ORCID ; Gisbert, Enric
KeywordsVitamin A
Thyroidal follicles
Skeletogenesis
Ossification
Nuclear receptors
Flatfish
Senegalese sole
Solea senegalensis
Issue Date30-Jun-2017
PublisherFrontiers Media
CitationFrontiers in Physiology 8: 458 (2017)
AbstractVitamin A (VA) and retinoid derivatives are known morphogens controlling vertebrate development. Despite the research effort conducted during the last decade, the precise mechanism of how VA induces post-natal bone changes, and particularly those operating through crosstalk with the thyroid hormones (THs) remain to be fully understood. Since effects and mechanisms seem to be dose and time-dependent, flatfish are an interesting study model as they undergo a characteristic process of metamorphosis driven by THs that can be followed by external appearance. Here, we studied the effects of VA imbalance that might determine Senegalese sole (Solea senegalensis) skeletogenetic phenotype through development of thyroid follicles, THs homeostasis and signaling when a dietary VA excess was specifically provided during pre-, pro- or post-metamorphic stages using enriched rotifers and Artemia as carriers. The increased VA content in enriched live prey was associated to a higher VA content in fish at all developmental stages. Dietary VA content clearly affected thyroid follicle development, T3 and T4 immunoreactive staining, skeletogenesis and mineralization in a dose and time-dependent fashion. Gene expression analysis showed that VA levels modified the mRNA abundance of VA- and TH-specific nuclear receptors at specific developmental stages. Present results provide new and key knowledge to better understand how VA and TH pathways interact at tissue, cellular and nuclear level at different developmental periods in Senegalese sole, unveiling how dietary modulation might determine juvenile phenotype and physiology.
Publisher version (URL)http://dx.doi.org/10.3389/fphys.2017.00458
URIhttp://hdl.handle.net/10261/168397
DOIhttp://dx.doi.org/10.3389/fphys.2017.00458
ISSN1664-042X
Appears in Collections:(IATS) Artículos
(ICMAN) Artículos
Files in This Item:
File Description SizeFormat 
flatfish_development_Fernandez.pdf4,13 MBAdobe PDFThumbnail
View/Open
Show full item record
Review this work
 

Related articles:


WARNING: Items in Digital.CSIC are protected by copyright, with all rights reserved, unless otherwise indicated.