English   español  
Por favor, use este identificador para citar o enlazar a este item: http://hdl.handle.net/10261/168042
COMPARTIR / IMPACTO:
Estadísticas
logo share SHARE logo core CORE   Add this article to your Mendeley library MendeleyBASE

Visualizar otros formatos: MARC | Dublin Core | RDF | ORE | MODS | METS | DIDL
Exportar a otros formatos:
Título

Phenotypic and lipidomic characterization of primary human epidermal keratinocytes exposed to simulated solar UV radiation

AutorDalmau, Núria; Andrieu-Abadie, Nathalie; Tauler, Romà; Bedia, Carmen
Palabras claveKeratinocyte
Keratinocyte differentiation
Untargeted lipidomics
UV irradiation
Fecha de publicación10-jul-2018
EditorElsevier
CitaciónJournal of Dermatological Science (2018)
ResumenBackground: Ultraviolet (UV) radiation is known to be one of the most important environmental hazards acting on the skin. The most part of UV radiation is absorbed in the epidermis, where keratinocytes are the most abundant and exposed cell type. Lipids have an important role in skin biology, not only for their important contribution to the maintenance of the permeability barrier but also for the production and storage of energy, membrane organization and cell signalling functions. However, the effects on the lipid composition of keratinocytes under UV radiation are little explored. Objective: The present work aims to explore the effects on the phenotype and lipid content of primary human keratinocytes exposed to simulated solar UV radiation. Methods: Keratinocytes were exposed to a single (acute exposure) and repeated simulated solar UV irradiations for 4 weeks (chronic exposure). Cell viability and morphology were explored, as well as the production of reactive oxygen species. Then, lipid extracts were analysed through liquid chromatography coupled to mass spectrometry (LC–MS) and the data generated was processed using the ROIMCR chemometric methodology together with partial least squares discriminant analysis (PLS-DA), to finally reveal the most relevant lipid changes that occurred in keratinocytes upon UV irradiation. Also, the potential induction of keratinocyte differentiation was explored by measuring the increase of involucrin. Results: Under acute irradiation, cell viability and morphology were not altered. However, a general increase of phosphatidylcholines (PC) phosphatidylethanolamines (PE) and phosphatidylglycerol (PG) together with a slight sphingomyelin (SM) decrease were found in UV irradiated cells, among other changes. In addition, keratinocyte cultures did not present any differentiation hallmark. Contrary to acute-irradiated cells, in chronic exposures, cell viability was reduced and keratinocytes presented an altered morphology. Also, hallmarks of differentiation, such as the increase of involucrin protein and the autophagy induction were detected. Among the main lipid changes that accompanied this phenotype, the increase of long-chain ceramides, lysoPC and glycerolipid species were found. Conclusion: Important lipid changes were detected under acute and chronic UV irradiation. The lipid profile under chronic exposure may represent a lipid fingerprint of the keratinocyte differentiation phenotype. © 2018
Versión del editorhttps://doi.org/10.1016/j.jdermsci.2018.07.002
URIhttp://hdl.handle.net/10261/168042
DOI10.1016/j.jdermsci.2018.07.002
Aparece en las colecciones: (IDAEA) Artículos
Ficheros en este ítem:
Fichero Descripción Tamaño Formato  
Phenotypic and lipidomic characterization of primary human epidermal keratinocytes exposed to simulated solar UV radiation.docx Embargado hasta 10 de julio de 2019941,9 kBMicrosoft Word XMLVisualizar/Abrir     Petición de una copia
Mostrar el registro completo
 

Artículos relacionados:


NOTA: Los ítems de Digital.CSIC están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.