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Título

MalR-mediated regulation of the Streptococcus pneumoniae malMP operon at promoter PM. Influence of a proximal divergent promoter region and competition between MalR and RNA polymerase proteins

AutorNieto, Concha ; Puyet, A.; Espinosa, Manuel
Palabras claveStaphylococcus-aureus
Escherichia-coli
Catabolite repression
Bacillus-subtilis
Open-complex
Transcription
DNA
Expression
Site
Inhibition
Fecha de publicación4-may-2001
EditorAmerican Society for Biochemistry and Molecular Biology
CitaciónThe Journal of Biological Chemistry 276, 14946-14954 (2001)
ResumenThe Streptococcus pneumoniae malregulon contains two operons, malXCD andmalMP involved in the uptake and utilization of maltosaccharides. Both operons are transcribed from two divergent promoters, PX andPM , and are negatively regulated by the MalR transcriptional repressor. Purified MalR protein binds to two DNA regions that encompasses both promoters, thus occupying its two operators, OM and OX. However, the levels of occupation and repression were different, being higher when MalR was bound to OM than when it was anchored to OX. Competition experiments between MalR and the Escherichia coli RNA polymerase on promoters PM andPX showed that the affinity of either protein for the promoter/operator DNA sequences was important to determine the frequency of transcription initiation. In addition to the control exerted by MalR, expression from promoter PM was affected by upstream sequences located within or close toPX promoter.
Descripción10 p.-7 fig.-1 tab.
Versión del editorhttps://doi.org/10.1074/jbc.M010911200
URIhttp://hdl.handle.net/10261/168011
DOI10.1074/jbc.M010911200
ISSN0021-9258
E-ISSN1083-351X
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