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Title

MalR-mediated regulation of the Streptococcus pneumoniae malMP operon at promoter PM. Influence of a proximal divergent promoter region and competition between MalR and RNA polymerase proteins

AuthorsNieto, Concha ; Puyet, A.; Espinosa, Manuel
KeywordsStaphylococcus-aureus
Escherichia-coli
Catabolite repression
Bacillus-subtilis
Open-complex
Transcription
DNA
Expression
Site
Inhibition
Issue Date4-May-2001
PublisherAmerican Society for Biochemistry and Molecular Biology
CitationThe Journal of Biological Chemistry 276, 14946-14954 (2001)
AbstractThe Streptococcus pneumoniae malregulon contains two operons, malXCD andmalMP involved in the uptake and utilization of maltosaccharides. Both operons are transcribed from two divergent promoters, PX andPM , and are negatively regulated by the MalR transcriptional repressor. Purified MalR protein binds to two DNA regions that encompasses both promoters, thus occupying its two operators, OM and OX. However, the levels of occupation and repression were different, being higher when MalR was bound to OM than when it was anchored to OX. Competition experiments between MalR and the Escherichia coli RNA polymerase on promoters PM andPX showed that the affinity of either protein for the promoter/operator DNA sequences was important to determine the frequency of transcription initiation. In addition to the control exerted by MalR, expression from promoter PM was affected by upstream sequences located within or close toPX promoter.
Description10 p.-7 fig.-1 tab.
Publisher version (URL)https://doi.org/10.1074/jbc.M010911200
URIhttp://hdl.handle.net/10261/168011
DOI10.1074/jbc.M010911200
ISSN0021-9258
E-ISSN1083-351X
Appears in Collections:(CIB) Artículos
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