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Biochemical profiling of rat embryonic stem cells grown on electrospun polyester fibers using synchrotron infrared microspectroscopy

AuthorsDoncel-Pérez, Ernesto CSIC ORCID; Ellis, Gary James CSIC ORCID ; Sandt, Christophe; Shuttleworth, Peter S. CSIC ORCID ; Bastida, Agatha CSIC ORCID ; Revuelta, Julia CSIC ORCID; García-Junceda, Eduardo CSIC ORCID ; Fernández-Mayoralas, Alfonso CSIC ORCID ; Garrido, Leoncio CSIC ORCID
FTIR spectroscopy
Neural progenitor cells
Issue Date2018
CitationAnalytical and Bioanalytical Chemistry 410: 3649-3660 (2018)
AbstractTherapeutic options for spinal cord injuries are severely limited; current treatments only offer symptomatic relief and rehabilitation focused on educating the individual on how to adapt to their new situation to make best possible use of their remaining function. Thus, new approaches are needed, and interest in the development of effective strategies to promote the repair of neural tracts in the central nervous system inspired us to prepare functional and highly anisotropic polymer scaffolds. In this work, an initial assessment of the behavior of rat neural progenitor cells (NPCs) seeded on poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) fiber scaffolds using synchrotron-based infrared microspectroscopy (SIRMS) is described. Combined with a modified touch imprint cytology sample preparation method, this application of SIRMS enabled the biochemical profiles of NPCs on the coated polymer fibers to be determined. The results showed that changes in the lipid and amide I–II spectral regions are modulated by the type and coating of the substrate used and the culture time. SIRMS studies can provide valuable insight into the early-stage response of NPCs to the morphology and surface chemistry of a biomaterial, and could therefore be a useful tool in the preparation and optimization of cellular scaffolds.
Publisher version (URL)
Identifiersdoi: 10.1007/s00216-018-1049-z
issn: 1618-2642
e-issn: 1618-2650
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