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Increased virulence in sunflower broomrape (Orobanche cumana Wallr.) populations from Southern Spain is associated with greater genetic diversity

AutorMartín-Sanz, Alberto; Lobo García de Vinuesa, Teresa; Rueda, Sandra; Pérez-Vich, Begoña ; Velasco Varo, Leonardo
Fecha de publicaciónjun-2017
Citación14th World Congress on Parasitic Plants (2017)
ResumenSunflower broomrape (Orobanche cumana Wallr.) is a holoparasitic weed that infects roots of sunflower in large areas of Europe and Asia. Two distant O. cumana gene pools have been identified in Spain, one in the Center (Cuenca province) and another one in the South (Guadalquivir Valley) of the country. Race F has been hypothesized to have arisen by separate mutational events in both gene pools. In Spain, race F spread in the middle 1990’s to become predominant and has been contained so far with resistant hybrids. Recently, enhanced virulent populations of O. cumana have been observed in commercial fields parasitizing race F resistant hybrids. From them, we collected 22 independent populations during 2014-2016 seasons, and conducted phenotypic and genetic diversity analysis. Virulence essays with Dupont Pioneer differential inbreds and hybrids and a set of hybrids widely used in Spain confirmed that the populations studied can parasitize most of the race F resistant hybrids tested. Consequently, they were characterized as race G. In parallel, a study with 20 broomrape populations from other countries from the Black Sea area, France and Hungary was conducted to compare the virulence pattern with the new Spanish populations. Surprisingly, some of the Spanish populations could not parasitize the line P96, which is a public differential line for race F, but they did to race G differential inbred and hybrids. Accordingly, the new populations have been classified as race GGV to distinguish them from other races G. Cluster analysis generated with molecular data from 20 high-quality SSR markers with a set of populations from the two Spanish gene pools and from other areas, mainly Eastern Europe, confirmed that race GGV populations maintain close genetic relatedness with the Guadalquivir Valley gene pool. This suggested that increased virulence was not caused by new introductions from other countries. Genetic diversity parameters revealed that the race GGV populations had much greater genetic diversity than conventional populations of the same area, containing only alleles present in the Guadalquivir Valley and Cuenca gene pools. The results suggested that increased virulence may have resulted from admixture of populations from the Guadalquivir Valley and Cuenca followed by recombination of avirulence genes. Ongoing studies are focused on genotyping more race GGV populations and using more markers to have a better comparison between inter- and intra- populations, and to try to get new insights in the race development process.
DescripciónTrabajo presentado en el 14th World Congress on Parasitic Plants (From genome to field), celebrado en Asilomar (California) el 24 y 25 de junio de 2017.
URIhttp://hdl.handle.net/10261/167961
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